Baird, Andrew

Breese, Virginia

Donahue, John E

Eliceiri, Brian

Gonzalez, Ana Maria

Jackson, Cynthia L

Johanson, Conrad E

Miller, Miles C

Podvin, Sonia

Stopa, Edward G

English

PubMed

Gonzalez Ana

Stopa Edward G

Jackson Cynthia L

Johanson Conrad E

Eliceiri Brian

Podvin Sonia

Breese Virginia

Miller Miles C

Donahue John E

Baird Andrew

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Cerebrospinal Fluid Research

ECRG-4 expression in normal and neoplastic choroid plexus

John E Donahue

Miles C Miller

Virginia Breese

Sonia Podvin

Brian Eliceiri

Cynthia L Jackson

Conrad E Johanson

Edward G Stopa

Ana Maria Gonzalez

Andrew Baird

Crossref

Ana Gonzalez

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ORAL PRESENTATION Open Access
ECRG-4 expression in normal and neoplastic
choroid plexus
John E Donahue1*, Miles C Miller1, Virginia Breese1, Sonia Podvin2, Brian Eliceiri2, Cynthia L Jackson1,
Conrad E Johanson3, Edward G Stopa1, Ana Maria Gonzalez4, Andrew Baird2
From 54th Annual Meeting of the Society for Research into Hydrocephalus and Spina Bifida
Vancouver, Canada. 7-10 July 2010
Background
The choroid plexus is a major site of gene expression of
esophageal cancer-related gene (ECRG)-4 during devel-
opment, suggesting that its gene product may be involved
in cerebrospinal fluid (CSF) homeostasis. Yet, ECRG-4 is
also a novel candidate tumor suppressor gene whose
expression is downregulated and is inversely associated
with a worse prognosis in several different cancers.
Reduced expression of ECRG-4 has been demonstrated
in most tumors, including colorectal carcinoma and
malignant glioma, to be mediated by hypermethylation of
its promoter.
Materials and methods
In this study, samples of normal human choroid plexus
(both fetal and adult) and choroid plexus neoplasms
(WHO grade I papilloma, grade II atypical papilloma,
and grade III carcinoma) were stained with antibodies
that we generated to augurin, the gene product of ECRG-
4. DNA was then extracted from the tissue, treated with
bisulfite, and subjected to PCR using a 217-base pair
region encompassing the ECRG-4 promotor to detect
methylation.
Results
Both fetal and adult human choroid plexus cells demon-
strated a robust positive immunostaining at the apical
surface that is consistent with our prior results in
human, rat, and mouse brains. In contrast, there was a
near-complete absence of immunostaining in all of the
choroid plexus neoplasms examined. The choroid plexus
carcinoma demonstrated significant methylation of the
ECRG-4 promotor region.
Conclusions
Taken together, these data suggest that ECRG-4 is down-
regulated in neoplasms of the choroid plexus just as has
been observed in other central nervous system (CNS) and
non-CNS cancers. This is likely due to hypermethylation
of the ECRG-4 promotor, as shown in the choroid plexus
carcinoma. Further analysis is underway to determine the
(1) physiologic and (2) pathophysiologic consequences of
ECRG-4 over- and under- expression in the choroid
plexus on CSF formation, function, and composition.
Author details
1Dept. of Pathology, Rhode Island Hospital/Alpert Medical School, 593 Eddy
St (APC 12115), Providence, RI 02903, USA. 2Dept. of Surgery, UCSD Medical
Center, 212 Dickinson St, CTFB310 MS 8296, San Diego, CA 92103, USA.
3Dept. of Neurosurgery, Rhode Island Hospital/Alpert Medical School, 593
Eddy St, Providence, RI 02903, USA. 4Neuropharmacology and Neurobiology
Section, School of Clinical and Experimental Medicine, College of Medical
and Dental Sciences, Institute of Biomedical Research (West), University of
Birmingham, Edgbaston, Birmingham B15 2TT, UK.
Published: 15 December 2010
doi:10.1186/1743-8454-7-S1-S32
Cite this article as: Donahue et al.: ECRG-4 expression in normal and
neoplastic choroid plexus. Cerebrospinal Fluid Research 2010 7(Suppl 1):S32.
* Correspondence: JDonahue3@Lifespan.org
1Dept. of Pathology, Rhode Island Hospital/Alpert Medical School, 593 Eddy
St (APC 12115), Providence, RI 02903, USA
Full list of author information is available at the end of the article
Donahue et al. Cerebrospinal Fluid Research 2010, 7(Suppl 1):S32
http://www.cerebrospinalfluidresearch.com/content/7/S1/S32
CEREBROSPINAL FLUID RESEARCH
© 2010 Donahue et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative
Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is properly cited.


http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3026510

ECRG-4 expression in normal and neoplastic choroid plexus

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