Carmona-Calero, Emilia M

Castañeyra-Perdomo, Agustin

Castañeyra-Ruiz, Agustin

Castañeyra-Ruiz, Leandro

Castro, Rafael

de Paz-Camona, Hector

Gonzalez-Marrero, Ibrahim

Gonzalez-Toledo, Juan M

English

PubMed

Agustin Castañeyra-Perdomo

Ibrahim Gonzalez-Marrero

Rafael Castro

Agustin Castañeyra-Ruiz

Hector de Paz-Camona

Leandro Castañeyra-Ruiz

Juan M Gonzalez-Toledo

Emilia M Carmona-Calero

Springer - Publisher Connector

ORAL PRESENTATION Open Access
Long-term effects of arterial hypertension on the
choroid plexus, blood to cerebrospinal fluid
barrier and CSF proteins and their involved in
certain types of dementia
Agustin Castañeyra-Perdomo1,2*, Ibrahim Gonzalez-Marrero1, Rafael Castro3, Agustin Castañeyra-Ruiz2,
Hector de Paz-Camona2, Leandro Castañeyra-Ruiz2, Juan M Gonzalez-Toledo1, Emilia M Carmona-Calero1,2
From 54th Annual Meeting of the Society for Research into Hydrocephalus and Spina Bifida
Vancouver, Canada. 7-10 July 2010
Background
The correlation of hypertension with vascular dementia
has long been established and it is becoming increasingly
clear that hypertension is also a reversible risk factor in
the development of Alzheimer’s dementia (AD). Aging,
AD and hypertension are major determinants of cogni-
tive dysfunction which are associated with profound
alterations in the structure and function of cerebral
blood vessels. The aim of the present work is to analyze
cerebrovascular blood flow in the middle cerebral artery
(MCA), the integrity of blood to CSF barrier (BCB), chor-
oid plexus (CP) structure and protein secretion as well as
cerebrospinal fluid (CSF) protein composition.
Materials and methods
Choroid plexus (CP), CSF and serum from hypertensive
(SHR) and control (WKY) rats of 8, 26 and 52 weeks of
age were used. Blood flow was measured by transcranial
Doppler sonography on MCA. Brain sections containing
CP were processed by immunohistochemistry using anti-
bodies against transthyretin (TTR), caspase-3 (CAS-3),
proliferating cell nuclear antigen (PCNA) and immuno-
globulin G (IGG). CSF from 26 weeks of age was used by
2D electrophoresis and western-blot.
Results
CP immunohistochemistry: TTR increased during aging
in SHR with respect to WKY. CAS-3 was undetectable
at 8 weeks in SHR and at 8 and 26 weeks in WKY, at
26 weeks the SHR showed a slight reaction which was
increased at 52 weeks of age, a soft reaction was also
observed at 52 weeks in WKY rats, SHR also expressed
a significant number of cells marked by CAS-3. PCNA
results were similar to CAS-3; IGG was mainly
expressed in the basolateral membrane of the CP and in
CP cells at 52 weeks of age in SHR. 2D electrophoresis
and Western-blot: By 2D, we found differences in the
CSF protein profile of the SHR when compared to CSF
of the WKY, these variations were similar to variations
found by other authors in AD. By Western-blot (WB)
the TTR was found to be higher in blood and lower in
the CSF of SHR with respect to WKY groups.
Conclusions
Long-term effects of hypertension cause a degeneration
and hypertrophy of the CP producing variations in pro-
tein secretion to the CSF that could influence the main-
tenance of neurons and functions in the brain.
Variations in CSF proteins in AD could similarly cause
a CP disruption and support the close connection
between cerebrovascular risk factors and AD in light of
the fact that they could share common pathogenic etio-
logical processes.
* Correspondence: acastane@ull.es
1Departamento de Anatomía, Facultad de Medicina, Universidad de La
Laguna, Tenerife, Spain
Full list of author information is available at the end of the article
Castañeyra-Perdomo et al. Cerebrospinal Fluid Research 2010, 7(Suppl 1):S29
http://www.cerebrospinalfluidresearch.com/content/7/S1/S29
CEREBROSPINAL FLUID RESEARCH
© 2010 Castañeyra-Perdomo et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the
Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is properly cited.
Author details
1Departamento de Anatomía, Facultad de Medicina, Universidad de La
Laguna, Tenerife, Spain. 2Departamento de Biotecnología, Instituto de
Investigación y Ciencias de Puerto del Rosario, Fuerteventura, Spain.
3Departamento de Fisiologia, Facultad de Medicina, Universidad de La
Laguna, Tenerife, Spain.
Published: 15 December 2010
doi:10.1186/1743-8454-7-S1-S29
Cite this article as: Castañeyra-Perdomo et al.: Long-term effects of
arterial hypertension on the choroid plexus, blood to cerebrospinal
fluid barrier and CSF proteins and their involved in certain types of
dementia. Cerebrospinal Fluid Research 2010 7(Suppl 1):S29.
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Castañeyra-Perdomo et al. Cerebrospinal Fluid Research 2010, 7(Suppl 1):S29
http://www.cerebrospinalfluidresearch.com/content/7/S1/S29
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Long-term effects of arterial hypertension on the choroid plexus, blood to cerebrospinal fluid barrier and CSF proteins and their involved in certain types of dementia

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Long-term effects of arterial hypertension on the choroid plexus, blood to cerebrospinal fluid barrier and CSF proteins and their involved in certain types of dementia

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