Gouya, Ghazaleh

Weisshaar, Stefan

Wolzt, Michael

BMC Pharmacology

English

PubMed

Stefan Weisshaar

Ghazaleh Gouya

Michael Wolzt

Springer - Publisher Connector

MEETING ABSTRACT Open Access
Thrombus formation is not pH-dependent
Stefan Weisshaar, Ghazaleh Gouya, Michael Wolzt*
From 16th Scientific Symposium of the Austrian Pharmacological Society (APHAR)
Vienna, Austria. 25-27 November 2010
Background
Experimental data have suggested that gastric acid
impairs clot formation and platelet aggregation, and sti-
mulates fibrinolysis. Thus, changing gastric pH to ≥ 6
may ameliorate acute gastrointestinal bleedings. The aim
of this study was to evaluate the impact of pH on clot
formation and stability in an ex vivo thrombus model.
Materials and methods
Venous blood from healthy subjects was perfused over
denuded porcine aorta at 5 mL/min at a shear rate of
212 sec−1 for thrombus formation. To evaluate the
impact of pH changes, a co-perfusion with different
acidic solutions was performed. Clot stability was evalu-
ated by incubation of the isolated thrombus with pH
solutions or gastric juice for up to four hours. The fibri-
nolytic activity was assessed by measuring D-dimer con-
centrations in the supernatant media. Thrombus size
was evaluated by measurement of D-dimer concentra-
tion of the plasmin-degraded thrombus.
Results
Co-perfusion with different pH solutions did not affect
thrombus size compared to control conditions. Clot
incubation at pH 3, 5 or 7 for up to 4 h did not increase
supernatant D-dimer concentrations. Incubation with
gastric juice at pH 5 for 4 h significantly elevated super-
natant D-dimer compared to pH 3 and pH 7 (0.09 ±
0.00 vs. 3.53 ± 4.90 vs. 0.14 ± 0.12 mg/dL).
Conclusions
Clot formation is unaltered by concomitant changes in
pH at low shear rate. Likewise, there is trivial if any
effect of pH or gastric juice on thrombus stability, indi-
cating that acute pharmacological modulation of gastric
pH might yield only little impact on thrombus formed
on gastrointestinal ulcers.
Published: 16 November 2010
doi:10.1186/1471-2210-10-S1-A43
Cite this article as: Weisshaar et al.: Thrombus formation is not
pH-dependent. BMC Pharmacology 2010 10(Suppl 1):A43.
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* Correspondence: michael.wolzt@meduniwien.ac.at
Department of Clinical Pharmacology, Medical University of Vienna, 1090
Vienna, Austria
Weisshaar et al. BMC Pharmacology 2010, 10(Suppl 1):A43
http://www.biomedcentral.com/1471-2210/10/S1/A43
© 2010 Wolzt et al; licensee BioMed Central Ltd.


Thrombus formation is not pH-dependent

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Thrombus formation is not pH-dependent

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