Barhoumi, M

Dotsika, E

Guizani, I

Koutsoni, O

BMC Proceedings

English

PubMed

O Koutsoni

M Barhoumi

I Guizani

E Dotsika

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LECTURE PRESENTATION Open Access
LiEIF and its recombinant polypeptides enhance
the maturation of mouse dendritic cells and the
production of the protective IL-12 cytokine
O Koutsoni1*, M Barhoumi2, I Guizani2, E Dotsika1
From Institut Pasteur International Network Annual Scientific Meeting
Hong Kong. 22-23 November 2010
Dendritic cells (DCs) maturation is associated with
upregulation of costimulatory molecules (CD80, CD86,
CD40) and secretion of cytokines including IL-12
which is important for generation of effective T cells.
Proteins of Leishmania parasite that stimulate the pro-
duction of IL-12 could be of significant interest either
as immunotherapeutic components for leishmaniasis or
as adjuvants. LeIF (Leishmania eukaryotic initiation
factor) belongs to this group of proteins since it
induces the production of IL-12, IL-10 and TNF-a by
human monocytes of healthy volunteers. In particular,
the induction of cytokines appears to be located in the
N-terminal part (1-226) of the protein. In the present
study we evaluated the ability of the recombinant pro-
tein L. infantum eIF (LieIF) and its constructs to
induce in vitro the maturation of myeloid DCs (mDCs)
and the production of cytokines supporting the polari-
zation of Th1 type immune response. For this purpose,
we used five synthetic peptides (16-18 aa) belonging in
the N-terminal region, eight overlapped recombinant
polypeptides covering the full protein sequence and
the full-length protein. Enriched mDCs were obtained
by ex vivo expansion of bone marrow cells, from
BALB/c mice, cultured with the hematopoietic factor
GM-CSF. The incubation of mDCs with the recombi-
nant polypeptides, led to their maturation since it was
observed a significant augmentation of the percentage
of mDCs that express the molecules CD40, CD80 and
CD86. On the contrary, the synthetic peptides did not
enhance CD40 and CD86 expression and drove only to
significant augmentation of CD80. In addition, we
evaluated the ability of LieIF and its polypeptides to
induce the production of IL-12, IL-10 and the expres-
sion of iNOS by mouse mDCs. We determined an
augmentation of the percentage of mDCs that produce
IL-12 upon their incubation with LieIF as well as with
all the recombinant polypeptides and with three syn-
thetic peptides whereas negligible amount of IL-10 was
obtained. Augmentation of iNOS was found after incu-
bation with the entire molecule of LieIF and with the
recombinant polypeptides. In conclusion, LieIF and
some of its recombinant polypeptides seem to have
immunomodulatory properties demonstrating their
potential use as therapeutic and prophylactic vaccine
antigens.
Author details
1Laboratory of Cellular Immunology, Department of Microbiology, Hellenic
Pasteur Institute, Athens 11521, Greece. 2Laboratoire d’Epidémiologie et
d’Ecologie Parasitaire, Institut Pasteur de Tunis, 1002 Tunis-Belvedère, Tunisia.
Published: 10 January 2011
doi:10.1186/1753-6561-5-S1-P41
Cite this article as: Koutsoni et al.: LiEIF and its recombinant
polypeptides enhance the maturation of mouse dendritic cells and the
production of the protective IL-12 cytokine. BMC Proceedings 2011 5
(Suppl 1):P41.
1Laboratory of Cellular Immunology, Department of Microbiology, Hellenic
Pasteur Institute, Athens 11521, Greece
Full list of author information is available at the end of the article
Koutsoni et al. BMC Proceedings 2011, 5(Suppl 1):P41
http://www.biomedcentral.com/1753-6561/5/S1/P41
© 2011 Koutsoni et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons
Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in
any medium, provided the original work is properly cited.


LiEIF and its recombinant polypeptides enhance the maturation of mouse dendritic cells and the production of the protective IL-12 cytokine

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LiEIF and its recombinant polypeptides enhance the maturation of mouse dendritic cells and the production of the protective IL-12 cytokine

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