C Jorgensen

F Apparailly

F Geissmann

FK Swirski

G Courties

J Presumey

L Ziegler-Heitbrock

English

PubMed

Springer - Publisher Connector

POSTER PRESENTATION Open Access
Myeloid cell subsets dynamic during progression
of mouse collagen-induced arthritis
J Presumey1,2*, C Jorgensen1,2,3, G Courties1,2, F Apparailly1,2,3
From 5th European Workshop on Immune-Mediated Inflammatory Diseases
Sitges-Barcelona, Spain. 1-3 December 2010
Introduction
Circulating monocytes consist at least of two main subsets
of immune cells arising from a common progenitor in the
bone marrow that can give rise to macrophages and den-
dritic cells (DCs) upon entry into tissues [1]. These cells
represent a prominent component of immune infiltrates
during the progression of immune-mediated inflammatory
disorders (IMID). Over the past years, multiple cell surface
markers have been used in attempt to define monocytes,
leading to the identification of several subpopulations. In
many models of infections and IMID, the so-called inflam-
matory monocytes CD11b+ Ly6C+ have been shown to
traffic to sites of inflammation and lymph nodes, differen-
tiating into inflammatory DCs in situ. A splenic “reservoir”
of monocytes able to migrate into injured tissues to regu-
late inflammation has also been recently described [3].
Aim
The present study aimed at better characterizing
monocytes subsets and monocyte-derived cells during
the development of autoimmune arthritis in mice.
Methods
Using flow cytometric immunophenotyping, we have
analyzed the expression of several specific myeloid cell
markers, CD11b, Ly6C, Ly6G, CD43, CD115, F4/80,
CD11c, PDCA1, CMHII during mouse collagen-induced
arthritis. We have specified the frequencies of the var-
ious cell subpopulations in the blood, spleen, draining
lymph nodes and inflamed joints at different stages of
the pathology, compared to naïve mice.
Results
As previously reported, percentages of whole blood
monocytes increased with disease progression from less
than 10% up to 25%. Locally, the number of neutrophils
increased from ~30% up to ~60% in the CIA joints com-
pared with steady state and represent the main source for
local TNF-a production. Surprisingly, monocytes are
found in significant amount within steady state joints
(~2%) and their number increased during CIA (~5% of
total cells). Finally, the subpopulation of Ly6Clow mono-
cytes observed in steady state conditions switched toward
Ly6Cint/high monocytes in CIA joints and produces high
levels of TNF-a, compared with naïve mice.
Conclusion
Overall, our data show that monocytes are not only
present within blood and lymphoid organs as previously
described, but also within joints, in both steady state
and pathological conditions. Although not fully differen-
tiated, these cells with inflammatory phenotype are
present within CIA joints.
Author details
1Inserm, U 844, Montpellier, France. 2Université Montpellier1, UFR de
Médecine, Montpellier, France. 3CHU Lapeyronie, Service Clinique d’Immuno-
Rhumatologie, Montpellier, France.
Published: 25 November 2010
References
1. Geissmann F, Manz MG, Jung S, Sieweke MH, Merad M, Ley K:
Development of monocytes, macrophages, and dendritic cells. Science
2010, 327(5966):656-61.
2. Ziegler-Heitbrock L, Ancuta P, Crowe S, Dalod M, Grau V, Hart DN, et al:
Nomenclature of monocytes and dendritic cells in blood. Blood 2010,
ahead of print.
3. Swirski FK, Nahrendorf M, Etzrodt M, Wildgruber M, Cortez-Retamozo V,
Panizzi P, et al: Identification of splenic reservoir monocytes and their
deployment to inflammatory sites. Science 2009, 325(5940):612-6.
doi:10.1186/1479-5876-8-S1-P54
Cite this article as: Presumey et al.: Myeloid cell subsets dynamic during
progression of mouse collagen-induced arthritis. Journal of Translational
Medicine 2010 8(Suppl 1):P54.
1Inserm, U 844, Montpellier, France
Full list of author information is available at the end of the article
Presumey et al. Journal of Translational Medicine 2010, 8(Suppl 1):P54
http://www.translational-medicine.com/content/8/S1/P54
© 2010 Presumey et al; licensee BioMed Central Ltd.


Myeloid cell subsets dynamic during progression of mouse collagen-induced arthritis

Crossref

POSTER PRESENTATION Open AccessMyeloid cell subsets dynamic during progressionof mouse collagen-induced arthritisJ Presumey1,2*, C Jorgensen1,2,3, G Courties1,2, F Apparailly1,2,3From 5th European Workshop on Immune-Mediated Inflammatory DiseasesSitges-Barcelona, Spain. 1-3 December 2010IntroductionCirculating monocytes consist at least of two main subsetsof immune cells arising from a common progenitor in thebone marrow that can give rise to macrophages and den-dritic cells (DCs) upon entry into tissues [1]. These cellsrepresent a prominent component of immune infiltratesduring the progression of immune-mediated inflammatorydisorders (IMID). Over the past years, multiple cell surfacemarkers have been used in attempt to define monocytes,leading to the identification of several subpopulations. Inmany models of infections and IMID, the so-called inflam-matory monocytes CD11b+ Ly6C+ have been shown totraffic to sites of inflammation and lymph nodes, differen-tiating into inflammatory DCs in situ. A splenic “reservoir”of monocytes able to migrate into injured tissues to regu-late inflammation has also been recently described [3].AimThe present study aimed at better characterizingmonocytes subsets and monocyte-derived cells duringthe development of autoimmune arthritis in mice.MethodsUsing flow cytometric immunophenotyping, we haveanalyzed the expression of several specific myeloid cellmarkers, CD11b, Ly6C, Ly6G, CD43, CD115, F4/80,CD11c, PDCA1, CMHII during mouse collagen-inducedarthritis. We have specified the frequencies of the var-ious cell subpopulations in the blood, spleen, draininglymph nodes and inflamed joints at different stages ofthe pathology, compared to naïve mice.ResultsAs previously reported, percentages of whole bloodmonocytes increased with disease progression from lessthan 10% up to 25%. Locally, the number of neutrophilsincreased from ~30% up to ~60% in the CIA joints com-pared with steady state and represent the main source forlocal TNF-a production. Surprisingly, monocytes arefound in significant amount within steady state joints(~2%) and their number increased during CIA (~5% oftotal cells). Finally, the subpopulation of Ly6Clow mono-cytes observed in steady state conditions switched towardLy6Cint/high monocytes in CIA joints and produces highlevels of TNF-a, compared with naïve mice.ConclusionOverall, our data show that monocytes are not onlypresent within blood and lymphoid organs as previouslydescribed, but also within joints, in both steady stateand pathological conditions. Although not fully differen-tiated, these cells with inflammatory phenotype arepresent within CIA joints.Author details1Inserm, U 844, Montpellier, France. 2Université Montpellier1, UFR deMédecine, Montpellier, France. 3CHU Lapeyronie, Service Clinique d’Immuno-Rhumatologie, Montpellier, France.Published: 25 November 2010References1. Geissmann F, Manz MG, Jung S, Sieweke MH, Merad M, Ley K:Development of monocytes, macrophages, and dendritic cells. Science2010, 327(5966):656-61.2. Ziegler-Heitbrock L, Ancuta P, Crowe S, Dalod M, Grau V, Hart DN, et al:Nomenclature of monocytes and dendritic cells in blood. Blood 2010,ahead of print.3. Swirski FK, Nahrendorf M, Etzrodt M, Wildgruber M, Cortez-Retamozo V,Panizzi P, et al: Identification of splenic reservoir monocytes and theirdeployment to inflammatory sites. Science 2009, 325(5940):612-6.doi:10.1186/1479-5876-8-S1-P54Cite this article as: Presumey et al.: Myeloid cell subsets dynamic duringprogression of mouse collagen-induced arthritis. Journal of TranslationalMedicine 2010 8(Suppl 1):P54.1Inserm, U 844, Montpellier, FranceFull list of author information is available at the end of the articlePresumey et al. Journal of Translational Medicine 2010, 8(Suppl 1):P54http://www.translational-medicine.com/content/8/S1/P54© 2010 Presumey et al; licensee BioMed Central Ltd.

Journal of Translational Medicine

Jorgensen C

Presumey J

Courties G

Apparailly F

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Development of monocytes, macrophages, and dendritic cells. Science

et al: Identification of splenic reservoir monocytes and their deployment to inflammatory sites. Science

et al: Nomenclature of monocytes and dendritic cells in blood. Blood 2010, ahead of print.

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Myeloid cell subsets dynamic during progression of mouse collagen-induced arthritis

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