A C-terminal region in Zds2p (ZH4) is required for regulation of Swe1p-dependent polarized cell growth and this region is necessary and sufficient for interaction with protein phosphatase 2A regulatory subunit, Cdc55p. Our results indicate that the Zds proteins regulate the Swe1p-dependent G2/M checkpoint in a CDC55-dependent manner

Adams A. E.

Altschul S. F.

Amon A.

Bandhakavi S.

Bi E.

Bialojan C.

Booher R. N.

Bucher N.

Chiroli E.

Collins S. R.

Dasso M.

David G. Drubin

Drees B. L.

Dunphy W. G.

Eichhorn P. J.

Enoch T.

Enserink J. M.

Estruch F.

Feven Tameire

Gauss R.

Gautier J.

Gavin A. C.

Ghaemmaghami S.

Gietz R. D.

Gould K. L.

Griffioen G.

Gueldener U.

Hartwell L. H.

Healy A. M.

Heo S. J.

Ho Y.

Hook S. S.

Hsu C. L.

Huh W. K.

James P.

Janssens V.

John S. W.

Keith G. Kozminski

Kimberly Yasutis

Kornitzer D.

Kozminski K. G.

Krogan N. J.

Kumagai A.

Larkin M. A.

Lew D. J.

Lobrich M.

Longtine M. S.

Lundgren K.

Lydall D.

Ma X. J.

Majka J.

Marissa Vignali

Matthew Ryder

McCleverty C. J.

McMillan J. N.

McNulty J. J.

Minshull J.

Miranda T. B.

Moreno S.

Notredame C.

Nurse P.

Ohnuki S.

Pal G.

Pierstorff E.

Queralt E.

Roy N.

Russell P.

Sayegh J.

Schwer B.

Sekiya-Kawasaki M.

Sherman F.

Shinohara M.

Shu Y.

Shubha A. Dighe

Sia R. A.

Sikorski R. S.

Sorger P. K.

Stanley Fields

Suganuma M.

Tanaka S.

Tong A. H.

Uetz P.

Wang Y.

Yakura M.

Yang H.

Yokoyama H.

Yu Y.

Zanelli C. F.

English

PubMed

Deletion of the paralogs ZDS1 and ZDS2 in the budding yeast Saccharomyces cerevisiae causes a mis-regulation of polarized cell growth. Here we show a function for these genes as regulators of the Swe1p (Wee1p) kinase–dependent G2/M checkpoint. We identified a conserved domain in the C-terminus of Zds2p consisting of amino acids 813–912 (hereafter referred to as ZH4 for Zds homology 4) that is required for regulation of Swe1p-dependent polarized bud growth. ZH4 is shown by protein affinity assays to be necessary and sufficient for interaction with Cdc55p, a regulatory subunit of protein phosphatase 2A (PP2A). We hypothesized that the Zds proteins are in a pathway that negatively regulates the Swe1p-dependent G2/M checkpoint via Cdc55p. Supporting this model, deletion of CDC55 rescues the aberrant bud morphology of a zds1Δzds2Δ strain. We also show that expression of ZDS1 or ZDS2 from a strong galactose-inducible promoter can induce mitosis even when the Swe1p-dependent G2/M checkpoint is activated by mis-organization of the actin cytoskeleton. This negative regulation requires the CDC55 gene. Together these data indicate that the Cdc55p/Zds2p module has a function in the regulation of the Swe1p-dependent G2/M checkpoint.</jats:p

Crossref

Molecular Biology of the Cell

Zds2p Regulates Swe1p-dependent Polarized Cell Growth in<i>Saccharomyces cerevisiae</i>via a Novel Cdc55p Interaction Domain

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http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3002390

Zds2p Regulates Swe1p-dependent Polarized Cell Growth in Saccharomyces cerevisiae via a Novel Cdc55p Interaction Domain

Zds2p Regulates Swe1p-dependent Polarized Cell Growth in Saccharomyces cerevisiae via a Novel Cdc55p Interaction Domain

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