Disclosed are methods of identifying subspecies of Cryptococcus flavescens and methods of treating or suppressing Fusarium head blight with the different Cryptococcus flavescens species. In particular, two genotypes, Genotypes A and B, were identified using the disclosed real time PCR technique. The following Cryptococcus flavescens strains were identified as being either Genotype A or B and as being able to suppress Fusarium head blight: NRRLY-7373, YB-601, YB-602, Y-7377, Y-7372, Y-7375, Y-7374, Y-7376, YB-328, Y-7379, and YB-744