Rubicon, a subunit of the Beclin 1-PI3-kinase complex and its homologue, PLEKHM1, negatively regulate endocytic pathway through the interaction with Rab7. Synchronous association with the Beclin 1–PI3-kinase complex and Rab7 is necessary for the function of Rubicon, but not PLEKHM1

Ayuko Sakane

Burda P.

Cao C.

Carlton J.

Choi J. H.

Christoforidis S.

Feng Y.

Gutierrez M. G.

Jager S.

James P.

Kabeya Y.

Keisuke Tabata

Kihara A.

Kohichi Matsunaga

Kurten R. C.

Liang C.

Liang X. H.

Matsunaga K.

Mizushima N.

Mukherjee S.

Ostermeier C.

Raiborg C.

Rojas R.

Seaman M.N.J.

Shah A. H.

Shaner N. C.

Simonsen A.

Stahl B.

Stein M. P.

Stenmark H.

Sun Q. M.

Suresh Subramani

Takeshi Noda

Takuya Sasaki

Tamotsu Yoshimori

Traer C. J.

Van Wesenbeeck L.

Xie Z. P.

Yoshimori T.

Zhao H.

Zhong Y.

English

PubMed

The endocytic and autophagic pathways are involved in the membrane trafficking of exogenous and endogenous materials to lysosomes. However, the mechanisms that regulate these pathways are largely unknown. We previously reported that Rubicon, a Beclin 1–binding protein, negatively regulates both the autophagic and endocytic pathways by unidentified mechanisms. In this study, we performed database searches to identify potential Rubicon homologues that share the common C-terminal domain, termed the RH domain. One of them, PLEKHM1, the causative gene of osteopetrosis, also suppresses endocytic transport but not autophagosome maturation. Rubicon and PLEKHM1 specifically and directly interact with Rab7 via their RH domain, and this interaction is critical for their function. Furthermore, we show that Rubicon but not PLEKHM1 uniquely regulates membrane trafficking via simultaneously binding both Rab7 and PI3-kinase.</jats:p

Crossref

Molecular Biology of the Cell

Rubicon and PLEKHM1 Negatively Regulate the Endocytic/Autophagic Pathway via a Novel Rab7-binding Domain

Adenovirus RID alpha regulates endosome maturation by mimicking GTP-Rab7.

Autophagic and tumour suppressor activity of a novel Beclin1-binding protein UVRAG.

Autophagosome formation: core machinery and adaptations.

Autophagy ﬁghts disease through cellular self-digestion.

Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with ;Mammalian Atg14 and UVRAG.

Beclin-phosphatidylinositol 3-kinase complex functions at the trans-Golgi network.

Beclin1-binding UVRAG targets the class C Vps complex to coordinate autophagosome maturation and endocytic trafﬁcking.

Coordination of membrane events during autophagy by multiple class III PI3-kinase complexes.

Distinct regulation of autophagic activity by

Downregulation of small GTPase Rab7 impairs osteoclast polarization and bone resorption.

Enhanced degradation of EGF receptors by a sorting nexin, SNX1.

Genomic libraries and a host strain designed for highly efﬁcient two-hybrid selection in yeast.

Identiﬁcation of Barkor as a mammalian autophagy-speciﬁc factor for Beclin 1 and class III phosphatidylinositol 3-kinase.

Improved monomeric red, orange and yellow ﬂuorescent proteins derived from Discosoma sp. red ﬂuorescent protein.

Induction of autophagy and inhibition of tumorigenesis by beclin 1.

Involvement of PLEKHM1 in osteoclastic vesicular transport and osteopetrosis in incisors absent rats and humans.

LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing.

Membrane recruitment of the cargo-selective retromer subcomplex is catalysed by the small GTPase Rab7 and inhibited by the Rab-GAP TBC1D5.

Myotubularin lipid phosphatase binds the hVPS15/hVPS34 lipid kinase complex on endosomes.

Puriﬁcation and identiﬁcation of novel Rab effectors using afﬁnity chromatography.

Rab GTPases as coordinators of vesicle trafﬁc.

Rab-7—-an important regulator of late endocytic membrane trafﬁc.

Rab3 reversibly recruits rabphilin to synaptic vesicles by a mechanism analogous to raf recruitment by ras.

Rab7 is required for the normal progression of the autophagic pathway in mammalian cells.

Regulation of retromer recruitment to endosomes by sequential action of Rab5 and Rab7.

Retromer function in endosome-to-Golgi retrograde transport is regulated by the yeast Vps34 PtdIns 3-kinase.

Role for Rab7 in maturation of late autophagic vacuoles.

SNX4 coordinates endosomal sorting of TfnR with dynein-mediated transport into the endocytic recycling compartment.

Sorting nexin 16 regulates EGF receptor trafﬁcking by phosphatidylinositol-3-phosphate interaction with the Phox domain.

Sorting nexin-1 mediates tubular endosome-to-TGN transport through coincidence sensing of high-curvature membranes and 3-phosphoinositides.

Structural basis of Rab effector speciﬁcity: crystal structure of the small G protein Rab3A complexed with the effector domain of rabphilin-3A.

The ESCRT machinery in endosomal sorting of ubiquitylated membrane proteins.

Toward unraveling membrane biogenesis in mammalian autophagy.

Two Beclin 1-binding proteins, Atg14L and Rubicon, reciprocally regulate autophagy at different stages.

Two distinct Vps34 phosphatidylinositol 3-kinase complexes function in autophagy and carboxypeptidase Y sorting in Saccharomyces cerevisiae.

http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2993745

Rubicon and PLEKHM1 Negatively Regulate the Endocytic/Autophagic Pathway via a Novel Rab7-binding Domain

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