HIA-a24 Gen Allele At Peripheral Blood Samples And Nasopharyngeal Cytobrush In Nasopharyngeal Carcinoma Patients In Makassar

Abstract

Nasopharyngeal cancer (NPC) is a malignancy that is highly associated with Epstein - Barr virus infection (EBV), highly oncogenic, easily transmitted through saliva and is a\ud multifactorial genetic disease with endemic character. EBV infection will cause an immune response in the patient themselves to recognize and eliminate foreign antigens which is associated with the ability of the antigen in presenting HLA molecules to cytotoxic T cells. Some of the genes under study which are genes that influence the occurrence of NPC are HLA genes. Aims: This study aims to determine the presence of the HLA- A24 allele in peripheral blood samples and nasopharyngeal cytobrush NPC patients in Makassar. Methods: The study design was a cross sectional analytic observational test of Fischer's Exact test on 21 samples of NPC patients who come in Dr. Wahidin Sudirohusodo Hospital. The examination was conducted by BOOM'S method for DNA isolation, and followed by PCR with primers forward and reverse HLA - A24. Result: The results showed the presence of the HLA allele A-24 in peripheral blood samples (37.5%) and nasopharyngeal cytobrush (42.9%) and stated that by performing the same method for taking specimen and examination, HLA-A24 existing in nasopharyngeal\ud cytobrush was also found in peripheral blood. Conclusion: It is concluded that the HLA A-24 was obtained in patients with NPC in Makassar