KD Gill

M Riemenschneider

PR Wenham

Ramesh Kandimalla

RC Petersen

SK Prabhakar

T Tapiola

English

PubMed

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MEETING ABSTRACT Open Access
CSF Potential Biomarkers Ab42 and Tau:
associations of Apo E Genotype
Ramesh Kandimalla1*, KD Gill1, SK Prabhakar2
From 1st International Congress on Neurobiology and Clinical Psychopharmacology and European
Psychiatric Association Conference on Treatment Guidance
Thessaloniki, Greece. 19-22 November 2009
Background
The most promising strategy to detect AD in preclinical
or presymptomatic stage need specific biomarkers. In
this study we elucidated the relationship between Apo E
genotype and CSF biomarkers Ab42 and Total tau in
Alzheimer’s Disease (AD) Patients, Non AD (NAD)
patients, Neurological controls (NCs) and Healthy
Controls (HCs).
Materials and methods
In this study we included 30 HC, 30 AD patients, 40
NAD, and 46 NC from Nehru Hospital, PGIMER, Chan-
digarh, India after obtaining informed consent from all
the subjects. Apo E Genotyping was done according to
the Wenham PR etal,1991. The levels of Ab42 and total
tau were determined by ELISA kits Innogenetics,
Belgium.
Results
Our data of CSF Ab42 and tau levels in conjunction
with ε4 allele had shown specificity and sensitivity of
100% and 42.8% respectively for the detection of AD.
Ab42 and Apo E ε4 combination had shown specificity
80.8% and sensitivity 72.1 %. The ε4 allele distribution
frequency was 40% and 2.5% in AD and NAD respec-
tively, where as ε4/4 genotype and ε3/4 genotype distri-
bution was 10 % and 50 % respectively. Our data has
shown that ε4 allele in combination with Ab42 to have
better sensitivity and specificity in the diagnosis of AD.
AD patients with at least one ε4 allele had significantly
lower CSF Ab42 levels than those without ε4 allele
(P < 0.001). There was a positive correlation of Ab42
with low MMSE scores.
Conclusions
Observation from our study suggest that decreased
Ab42 and increased tau level in CSF along with Apo E
ε4 allele as risk factors for AD. Our study also shows ε4
allele incidence to be a risk factor for AD.
Acknowledgements
ICMR, New Delhi, India for funding this study and Senior Residents of
department of neurology. Colleagues Nidhi, Deep raj and Aditya
Author details
1Department of Biochemistry, PGIMER, Chandigarh, India. 2Department of
Neurology, PGIMER, Chandigarh, India.
Published: 22 April 2010
References
1. Wenham PR, Price WH, Blandell G: Apolipoprotein E genotyping by one-
stage PCR. Lancet 1991, 337:1158-9.
2. Tapiola T, Pirttila T, Mehta PD, Alafuzofff I, Lehtovirta M, Soininen H:
Relationship between apoE genotype and CSF beta-amyloid (1-42) and
tau in patients with probable and definite Alzheimer’s disease. Neurobiol
Aging 2000, 2:735-40.
3. Riemenschneider M, Schmolke M, Lautenschlager N, Vanderstichele H,
Vanmechelen E, Guder WG, et al: Association of CSF apolipoprotein E,
Abeta42 and cognition in Alzheimer’s disease. Neurobiol Aging 2002,
23:205-11.
4. Petersen RC, Smith GE, Ivnik RJ, Tangalos EG, Schaid DJ, Thibodeau SN,
et al: Apolipoprotein E status as a predictor of the development of
Alzheimer’s disease in memory-impaired individuals. JAMA 1995,
273:1274-8.
doi:10.1186/1744-859X-9-S1-S104
Cite this article as: Kandimalla et al.: CSF Potential Biomarkers Ab42 and
Tau: associations of Apo E Genotype. Annals of General Psychiatry 2010
9(Suppl 1):S104.
1Department of Biochemistry, PGIMER, Chandigarh, India
Kandimalla et al. Annals of General Psychiatry 2010, 9(Suppl 1):S104
http://www.annals-general-psychiatry.com/content/9/S1/S104
© 2009 Kandimalla et al; licensee BioMed Central Ltd.


CSF Potential Biomarkers Aβ42 and Tau: associations of Apo E Genotype

Gill KD

Kandimalla Ramesh

Prabhakar SK

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Annals of General Psychiatry

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Apolipoprotein E genotyping by onestage PCR. Lancet

Apolipoprotein E status as a predictor of the development of Alzheimer’s disease in memory-impaired individuals.

Relationship between apoE genotype and CSF beta-amyloid (1-42) and tau in patients with probable and definite Alzheimer’s disease. Neurobiol Aging

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CSF Potential Biomarkers Aβ42 and Tau: associations of Apo E Genotype

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