本研究以超音波萃取及管柱層析搭配超臨界流體抗溶結晶程序，於寧夏枸杞果粒中萃取純化出生化活性物質玉米黃素酯，並製備高純度抗氧化奈米粉粒體。首先，經管柱層析純化超音波丙酮萃取物，接續乙醇液態抗溶二氯甲烷溶液，單離出98.3 %玉米黃素酯。另外，以管柱層析超音波攪拌丙酮萃出物，結合超臨界二氧化碳抗溶結晶乙酸乙酯溶液，獲得玉米黃素酯的濃度可由72.75 mg/g 提升至931.5 mg/g。乙醇液態抗溶結晶玉米黃素酯的回收率為25.2 %，超臨界二氧化碳抗溶結晶的回收率可達到64.9 %。超臨界二氧化碳抗溶結晶實驗設計的結果顯示，進料濃度及進料流速，是影響沉澱物中玉米黃素酯純度及超臨界二氧化碳抗溶結晶產生葉狀奈米顆粒主要因子。此高純度的玉米黃素酯溶於含有二甲基亞碸水溶液，經體外細胞實驗顯示仍具有保護人類視網膜色素上皮細胞被氧化傷害的能力(存活率由22 %增加到31%)；與抗氧化活性極高的玉米黃素(存活率由43 %增加到94 %)相比，效果較低。
英文摘要
This study employed ultrasonic extraction, column elution fractionation and supercritical carbon dioxide anti-solvent recrystallization in preparing the pured nano-sized zeaxanthin dipalmitate (ZDP) from Lycium barbarum fruits. Column fractionation of ultrasonic acetone extract coupled with supercritical carbondioxide anti-ethyl acetate solution ehanced amount of ZDP from 72.75 mg/g in the acetone extract to 931.5 mg/g in the SAS precipitates. The ZDP recovered at 25.2% and 64.9 % from Lycium barbarum fruits individually by liquid anti-solvent and by supercritical anti-solvent recrystallization. Experimental design by response surface method showed that feed concentration and feed flow rate are important on the purification and the preparation of leaf type nano-sized ZDP containing particles. These purified ZDP precipitates in aqueous dimethyl sulfoxide solution exhibited anti-oxidative abilities on proliferating human adult retinal pigment epithelial cells (viability from 22% to 31%), though it was a meager increase compared to those of zeaxanthin (viability from 43% to 94%)
