長喙殼菌類真菌為世界上最重要之林木病害來源，包括了Ophiostoma及Ceratocysts屬及其無性繁殖態，為防範此類病原真菌藉由木材輸入而危害我國之森林原生樹種，並評估此類病原之風險，本計劃將針對進口木材及本土林木中長喙殼菌類真菌進行偵測，建立菌種形態特性及DNA序列資料庫，提供快速且正確檢測木材中此類有害真菌之基礎。除此之外，櫟樹猝死病菌亦是當前世界重要之森林病菌，本研究將針對其可能藉木材輸入之傳播途徑進行監測，以杜絕其危害。潛在病原菌之偵測及鑑定包括形態及分子序列方式，依據不同之菌種類別之特性，以特有之方式進行菌種分離，針對特定rDNA片段進行解序，建立形態及分子鑑定標準，有助於發展快速之檢測方法，攔截有害病原之輸入。Ophiostomatoid fungi are the most important forest pathogens in the world. Species of Ophiostoma and Ceratocystis and their anamorph genera are collectively referred to as the ophiostomatoid fungi. To prevent the introduction of these pathogens from plaguing our natural trees, and to evaluate their risk, the aims of this project is to inspect ophiostomatoid fungi on wood which is from importation or from indigenous plants. Morphological characteristics and DNA sequences of these fungi can be established to provide the basis for their accurate and quick detection. Additionally, Sudden Oak Death, caused by Phytophthora ramorum, is currently a serious problem in many countries. This pathogen will also be the major target in our inpection. Both methods of morphology identification and DNA sequence comparison will be applied. Specific approaches of isolation will be adopted to obtain specific groups of fungi. Specific primers are used to amplify specific DNA fragments for comparisons with DNA sequences deposited in GenBank. Based on the morphological and molecular data acquired, it can be expected that these potential pathogens can be warded off from our country
