Background: Endurance exercise induces lipolysis, increases circulating concentrations of free fatty acids (FFA) and the uptake and oxidation of fatty acids in the working muscle. Less is known about the regulation of lipid metabolism in the liver during and post-exercise

A Andersson

A Peter

A Petridou

AS Leon

B Burguera

C Fernandez

C Hu

C Simonelli

C Thamer

CE Mondon

Chunxiu Hu

Cora Weigert

DE Larson-Meyer

DE Vance

E Borsheim

E Cullinane

Erwin Schleicher

F Magkos

G Ahlborg

G Annuzzi

G Schoiswohl

G van

GG Ziogas

Guowang Xu

Hans U. Häring

HB Nielsen

HE Aldred

J Gorski

J Triscari

JK Reddy

JM Gill

JO Holloszy

L Eriksson

LL Listenberger

Lorraine Brennan

M Hoene

MD Jensen

Miriam Hoene

MS Gauthier

NA Johnson

Rainer Lehmann

RS Rector

S Bijlsma

S Schafer

S Wiklund

SL Herd

SP Sady

TW Mitchell

VJ Vieira

X Han

Xianlin Han

Xinjie Zhao

Y Tamura

YE Tsekouras

ZY Chen

ZZ Li

English

PubMed

Hu, Chunxiu

Hoene, Miriam

Zhao, Xinjie

Haring, Hans U

Schleicher, Erwin

Lehmann, Rainer

Han, Xianlin

Xu, Guowang

Weigert, Cora

Digital Commons@Becker

Lipidomics analysis reveals efficient storage of hepatic triacylglycerides enriched in unsaturated fatty acids after one bout of exercise in mice

Haering, Hans U.

Institutional Repository of Dalian Institute of Chemical Physics, CAS

Lipidomics Analysis Reveals Efficient Storage of Hepatic Triacylglycerides Enriched in Unsaturated Fatty Acids after One Bout of Exercise in Mice

BACKGROUND: Endurance exercise induces lipolysis, increases circulating concentrations of free fatty acids (FFA) and the uptake and oxidation of fatty acids in the working muscle. Less is known about the regulation of lipid metabolism in the liver during and post-exercise. METHODOLOGY/PRINCIPAL FINDINGS: We performed an ultra fast liquid chromatography-mass spectrometry (UFLC-MS) based lipidomics analysis of liver tissue samples obtained from C57Bl/6J mice immediately after a 60 min treadmill run of moderate intensity, and after 3 h of recovery. The PLS-DA scores plot for 115 quantified lipid molecular species revealed a clear separation of the hepatic lipid profile of sedentary from recovering mice, but not from mice immediately after running. 21 lipid species were considered to be most responsible for the difference in the hepatic lipid profiles, including 17 triacylglycerides (TG), one lysophosphatidylcholine (LPC) and three phosphatidylcholines (PC). TG species were found to be more abundant in the recovery phase, while PC species were decreased. The degree of accumulation of individual TG species correlated well with the amount of theoretical energy stored whereas no increase was found for TG species containing only saturated or one monounsaturated fatty acid. Total liver TG content as assayed by an enzymatic method was increased to 163% in the recovery phase, while it was significantly decreased in skeletal muscle by the exercise bout and remained less in the recovery phase. Results from fasted and refed mice indicate that fasting-induced lipolysis was associated with a pronounced accumulation of hepatic TG, which is reversed by refeeding for 5 h. Thus food intake per se did not elevate hepatic TG. CONCLUSION: These data indicate that high availability of FFA induced by endurance exercise or fasting resulted in a transient hepatic TG accumulation, while muscle TG content was decreased during exercise presumably due to increased muscle fatty acid oxidation

Hans U Häring

Directory of Open Access Journals

PLoS ONE

Lipidomics analysis reveals efficient storage of hepatic triacylglycerides enriched in unsaturated fatty acids after one bout of exercise in mice.

Endurance exercise induces lipolysis, increases circulating concentrations of free fatty acids (FFA) and the uptake and oxidation of fatty acids in the working muscle. Less is known about the regulation of lipid metabolism in the liver during and post-exercise.We performed an ultra fast liquid chromatography-mass spectrometry (UFLC-MS) based lipidomics analysis of liver tissue samples obtained from C57Bl/6J mice immediately after a 60 min treadmill run of moderate intensity, and after 3 h of recovery. The PLS-DA scores plot for 115 quantified lipid molecular species revealed a clear separation of the hepatic lipid profile of sedentary from recovering mice, but not from mice immediately after running. 21 lipid species were considered to be most responsible for the difference in the hepatic lipid profiles, including 17 triacylglycerides (TG), one lysophosphatidylcholine (LPC) and three phosphatidylcholines (PC). TG species were found to be more abundant in the recovery phase, while PC species were decreased. The degree of accumulation of individual TG species correlated well with the amount of theoretical energy stored whereas no increase was found for TG species containing only saturated or one monounsaturated fatty acid. Total liver TG content as assayed by an enzymatic method was increased to 163% in the recovery phase, while it was significantly decreased in skeletal muscle by the exercise bout and remained less in the recovery phase. Results from fasted and refed mice indicate that fasting-induced lipolysis was associated with a pronounced accumulation of hepatic TG, which is reversed by refeeding for 5 h. Thus food intake per se did not elevate hepatic TG.These data indicate that high availability of FFA induced by endurance exercise or fasting resulted in a transient hepatic TG accumulation, while muscle TG content was decreased during exercise presumably due to increased muscle fatty acid oxidation

Hu Chunxiu

Hoene Miriam

Zhao Xinjie

Häring Hans U.

Schleicher Erwin

Lehmann Rainer

Han Xianlin

Xu Guowang

Weigert Cora

Public Library of Science (PLOS)

Crossref

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http://digitalcommons.wustl.edu/cgi/viewcontent.cgi?article=1732&context=open_access_pubs

Lipidomics Analysis Reveals Efficient Storage of Hepatic Triacylglycerides Enriched in Unsaturated Fatty Acids after One Bout of Exercise in Mice

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Digital Commons@Becker

Institutional Repository of Dalian Institute of Chemical Physics, CAS

Directory of Open Access Journals

Public Library of Science (PLOS)

Crossref