A new software package allows for dense electron microscopy reconstructions of neuronal networks in the fruit fly brain, and reveals specific differences in microcircuits between insects and vertebrates

A May

A Ramaekers

A Younossi-Hartenstein

A. H Brand

A. H Watson

Albert Cardona

Anchi Cheng

B Schmid

B Ye

B. H Peters

B. L Chen

B. W Strowbridge

Benjamin Schmid

C Larsen

C Nassif

C Suloway

C. J Feeney

D Schubert

D. G Lowe

D. R Nässel

E. L Johnson

F Leiss

G Knott

G Silberberg

H Shibasaki

I. A Meinertzhagen

J Schindelin

J Song

J. C Fiala

J. G White

J. H Park

J. R Anderson

J. R Kremer

J. W Truman

Jim Pulokas

K Dumstrei

K Yasuyama

Kristen M. Harris

L Williams

L. S Krimer

M Hortsch

M Reigl

M. C Stone

M. M Rolls

M. W Dodson

N Kalisman

P. G Distler

Pavel Tomancak

R. J Cauchi

R. J Douglas

R. J Kittel

S Doronkin

S Saalfeld

S Schaefer

S. E Fahrbach

Stephan Preibisch

Stephan Saalfeld

U Alon

V Braitenberg

V Hartenstein

V Kaynig

V. L Cropley

Volker Hartenstein

W Denk

W Pereanu

Y Mishchenko

Y Zheng

Z. M Lasiecka

PLoS Biology

English

PubMed

The analysis of microcircuitry (the connectivity at the level of individual neuronal processes and synapses), which is indispensable for our understanding of brain function, is based on serial transmission electron microscopy (TEM) or one of its modern variants. Due to technical limitations, most previous studies that used serial TEM recorded relatively small stacks of individual neurons. As a result, our knowledge of microcircuitry in any nervous system is very limited. We applied the software package TrakEM2 to reconstruct neuronal microcircuitry from TEM sections of a small brain, the early larval brain of Drosophila melanogaster. TrakEM2 enables us to embed the analysis of the TEM image volumes at the microcircuit level into a light microscopically derived neuro-anatomical framework, by registering confocal stacks containing sparsely labeled neural structures with the TEM image volume. We imaged two sets of serial TEM sections of the Drosophila first instar larval brain neuropile and one ventral nerve cord segment, and here report our first results pertaining to Drosophila brain microcircuitry. Terminal neurites fall into a small number of generic classes termed globular, varicose, axiform, and dendritiform. Globular and varicose neurites have large diameter segments that carry almost exclusively presynaptic sites. Dendritiform neurites are thin, highly branched processes that are almost exclusively postsynaptic. Due to the high branching density of dendritiform fibers and the fact that synapses are polyadic, neurites are highly interconnected even within small neuropile volumes. We describe the network motifs most frequently encountered in the Drosophila neuropile. Our study introduces an approach towards a comprehensive anatomical reconstruction of neuronal microcircuitry and delivers microcircuitry comparisons between vertebrate and insect neuropile

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An integrated micro- and macroarchitectural analysis of the Drosophila brain by computer-assisted serial section electron microscopy.

The analysis of microcircuitry (the connectivity at the level of individual neuronal processes and synapses), which is indispensable for our understanding of brain function, is based on serial transmission electron microscopy (TEM) or one of its modern variants. Due to technical limitations, most previous studies that used serial TEM recorded relatively small stacks of individual neurons. As a result, our knowledge of microcircuitry in any nervous system is very limited. We applied the software package TrakEM2 to reconstruct neuronal microcircuitry from TEM sections of a small brain, the early larval brain of Drosophila melanogaster. TrakEM2 enables us to embed the analysis of the TEM image volumes at the microcircuit level into a light microscopically derived neuro-anatomical framework, by registering confocal stacks containing sparsely labeled neural structures with the TEM image volume. We imaged two sets of serial TEM sections of the Drosophila first instar larval brain neuropile and one ventral nerve cord segment, and here report our first results pertaining to Drosophila brain microcircuitry. Terminal neurites fall into a small number of generic classes termed globular, varicose, axiform, and dendritiform. Globular and varicose neurites have large diameter segments that carry almost exclusively presynaptic sites. Dendritiform neurites are thin, highly branched processes that are almost exclusively postsynaptic. Due to the high branching density of dendritiform fibers and the fact that synapses are polyadic, neurites are highly interconnected even within small neuropile volumes. We describe the network motifs most frequently encountered in the Drosophila neuropile. Our study introduces an approach towards a comprehensive anatomical reconstruction of neuronal microcircuitry and delivers microcircuitry comparisons between vertebrate and insect neuropile.ISSN:1544-9173ISSN:1545-788

Cardona, Albert

Saalfeld, Stephan

Preibisch, Stephan

Schmid, Benjamin

Cheng, Anchi

Pulokas, Jim

Tomancak, Pavel

Hartenstein, Volker

Repository for Publications and Research Data

An Integrated Micro- and Macroarchitectural Analysis of the Drosophila Brain by Computer-Assisted Serial Section Electron Microscopy

Preibisch, Stephan W.

Tomancák, Pavel

Max Planck Society eDoc Server

The analysis of microcircuitry (the connectivity at the level of individual neuronal processes and synapses), which is indispensable for our understanding of brain function, is based on serial transmission electron microscopy (TEM) or one of its modern variants. Due to technical limitations, most previous studies that used serial TEM recorded relatively small stacks of individual neurons. As a result, our knowledge of microcircuitry in any nervous system is very limited. We applied the software package TrakEM2 to reconstruct neuronal microcircuitry from TEM sections of a small brain, the early larval brain of Drosophila melanogaster. TrakEM2 enables us to embed the analysis of the TEM image volumes at the microcircuit level into a light microscopically derived neuro-anatomical framework, by registering confocal stacks containing sparsely labeled neural structures with the TEM image volume. We imaged two sets of serial TEM sections of the drosophila first instar larval brain neuropile and one ventral nerve cord segment, and here report our first results pertaining to Drosophila brain microcircuitry. Terminal neurites fall into a small number of generic classes termed globular, varicose, axiform, and dendritiform. Globular and varicose neurites have large diameter segments that carry almost exclusively presynaptic sites. Dendritiform neurites are thin, highly branched processes that are almost exclusively postsynaptic. Due to the high branching density of dendritiform fibers and the fact that synapses are polyadic, neurites are highly interconnected even within small neuropile volumes. We describe the network motifs most frequently encountered in the drosophila neuropile. Our study introduces an approach towards a comprehensive anatomical reconstruction of neuronal microcircuitry and delivers microcircuitry comparisons between vertebrate and insect neuropile

Cardona, A.

Saalfeld, S.

Preibisch, S.

Schmid, B.

Cheng, A.

Pulokas, J.

Tomancak, P.

Hartenstein, V.

MDC Repository

An integrated micro- and macroarchitectural analysis of the drosophila brain by computer-assisted serial section electron microscopy

Tomancák, P.

MPG.PuRe

Crossref

Cardona, A

Saalfeld, S

Preibisch, S

Schmid, B

Cheng, A

Pulokas, J

Tomancak, P

Hartenstein, V

ZORA

Cardona Albert

Saalfeld Stephan

Preibisch Stephan

Schmid Benjamin

Cheng Anchi

Pulokas Jim

Tomancak Pavel

Hartenstein Volker

Public Library of Science (PLOS)

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An Integrated Micro- and Macroarchitectural Analysis of the Drosophila Brain by Computer-Assisted Serial Section Electron Microscopy

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