Programmed cell death (PCD) is a necessary part of the life of multi-cellular organisms. A type of plant PCD is the defensive hypersensitive response (HR) elicited via recognition of a pathogen by host resistance (R) proteins. The lethal, recessive accelerated cell death 11 (acd11) mutant exhibits HR-like accelerated cell death, and cell death execution in acd11 shares genetic requirements for HR execution triggered by one subclass of R proteins

A Kosters

AS Murphy

B Pinan-Lucarre

Berthe Katrine Fiil

BJ Feys

Cyril Zipfel

D Best

D Hofius

D Mackey

Daniel Hofius

EH Baehrecke

EM Gilroy

F Geu-Flores

FH Wu

Frederikke G. Malinovsky

GI Jurkowski

H Kim

H Liang

H Nakashita

H. Bjørn Nielsen

J Akaishi

J Koga

J Muller

JD Jones

JM Alonso

John Mundy

JR Peart

JS Bonifacino

JT Greenberg

Lea Vig McKinney

M Bartsch

M Leist

MA Torres

Martina Beck

MJ Axtell

Mohammed Bendahmane

Morten Petersen

N Aarts

N Geldner

N Hatsugai

P Brodersen

P Tornero

Peter Brodersen

R Schwacke

RA Irizarry

Silke Robatzek

SJ Clough

Stefano Pietra

Stephan Thorgrimsen

T Hruz

T Tzfira

TK Eitas

WJ Lemon

Y Takahashi

Y Zhang

PLoS ONE

English

PubMed

BackgroundProgrammed cell death (PCD) is a necessary part of the life of multi-cellular organisms. A type of plant PCD is the defensive hypersensitive response (HR) elicited via recognition of a pathogen by host resistance (R) proteins. The lethal, recessive accelerated cell death 11 (acd11) mutant exhibits HR-like accelerated cell death, and cell death execution in acd11 shares genetic requirements for HR execution triggered by one subclass of R proteins.Methodology/principal findingsTo identify genes required for this PCD pathway, we conducted a genetic screen for suppressors of acd11, here called lazarus (laz) mutants. In addition to known suppressors of R protein-mediated HR, we isolated 13 novel complementation groups of dominant and recessive laz mutants. Here we describe laz1, which encodes a protein with a domain of unknown function (DUF300), and demonstrate that LAZ1 contributes to HR PCD conditioned by the Toll/interleukin-1 (TIR)-type R protein RPS4 and by the coiled-coil (CC)-type R protein RPM1. Using a yeast-based topology assay, we also provide evidence that LAZ1 is a six transmembrane protein with structural similarities to the human tumor suppressor TMEM34. Finally, we demonstrate by transient expression of reporter fusions in protoplasts that localization of LAZ1 is distributed between the cytosol, the plasma membrane and FM4-64 stained vesicles.Conclusions/significanceOur findings indicate that LAZ1 functions as a regulator or effector of plant PCD associated with the HR, in addition to its role in acd11-related death. Furthermore, the similar topology of a plant and human DUF300 proteins suggests similar functions in PCD across the eukaryotic kingdoms, although a direct role for TMEM34 in cell death control remains to be established. Finally, the subcellular localization pattern of LAZ1 suggests that it may have transport functions for yet unknown, death-related signaling molecules at the plasma membrane and/or endosomal compartments. In summary, our results validate the utility of the large-scale suppressor screen to identify novel components with functions in plant PCD, which may also have implications for deciphering cell death mechanisms in other organisms

Frederikke G Malinovsky

H Bjørn Nielsen

Directory of Open Access Journals

Lazarus1, a DUF300 protein, contributes to programmed cell death associated with Arabidopsis acd11 and the hypersensitive response.

 shares genetic requirements for HR execution triggered by one subclass of R proteins. which encodes a protein with a domain of unknown function (DUF300), and demonstrate that LAZ1 contributes to HR PCD conditioned by the Toll/interleukin-1 (TIR)-type R protein RPS4 and by the coiled-coil (CC)-type R protein RPM1. Using a yeast-based topology assay, we also provide evidence that LAZ1 is a six transmembrane protein with structural similarities to the human tumor suppressor TMEM34. Finally, we demonstrate by transient expression of reporter fusions in protoplasts that localization of LAZ1 is distributed between the cytosol, the plasma membrane and FM4–64 stained vesicles.-related death. Furthermore, the similar topology of a plant and human DUF300 proteins suggests similar functions in PCD across the eukaryotic kingdoms, although a direct role for TMEM34 in cell death control remains to be established. Finally, the subcellular localization pattern of LAZ1 suggests that it may have transport functions for yet unknown, death-related signaling molecules at the plasma membrane and/or endosomal compartments. In summary, our results validate the utility of the large-scale suppressor screen to identify novel components with functions in plant PCD, which may also have implications for deciphering cell death mechanisms in other organisms

Malinovsky Frederikke G.

Brodersen Peter

Fiil Berthe Katrine

McKinney Lea Vig

Thorgrimsen Stephan

Beck Martina

Nielsen H. Bjørn

Pietra Stefano

Zipfel Cyril

Robatzek Silke

Petersen Morten

Hofius Daniel

Mundy John

Public Library of Science (PLOS)

 and the Hypersensitive Response

Crossref

Malinovsky, Frederikke G

Brodersen, Peter

Fiil, Berthe Katrine

McKinney, Lea Vig

Thorgrimsen, Stephan

Beck, Martina

Nielsen, H Bjørn

Pietra, Stefano

Zipfel, Cyril

Robatzek, Silke

Petersen, Morten

Hofius, Daniel

Mundy, John

Copenhagen University Research Information System

Lazarus1, a DUF300 protein, contributes to programmed cell death associated with Arabidopsis acd11 and the hypersensitive response

Background: Programmed cell death (PCD) is a necessary part of the life of multi-cellular organisms. A type of plant PCD is the defensive hypersensitive response (HR) elicited via recognition of a pathogen by host resistance (R) proteins. The lethal, recessive accelerated cell death 11 (acd11) mutant exhibits HR-like accelerated cell death, and cell death execution in acd11 shares genetic requirements for HR execution triggered by one subclass of R proteins. Methodology/Principal Findings: To identify genes required for this PCD pathway, we conducted a genetic screen for suppressors of acd11, here called lazarus (laz) mutants. In addition to known suppressors of R protein-mediated HR, we isolated 13 novel complementation groups of dominant and recessive laz mutants. Here we describe laz1, which encodes a protein with a domain of unknown function (DUF300), and demonstrate that LAZ1 contributes to HR PCD conditioned by the Toll/interleukin-1 (TIR)-type R protein RPS4 and by the coiled-coil (CC)-type R protein RPM1. Using a yeast-based topology assay, we also provide evidence that LAZ1 is a six transmembrane protein with structural similarities to the human tumor suppressor TMEM34. Finally, we demonstrate by transient expression of reporter fusions in protoplasts that localization of LAZ1 is distributed between the cytosol, the plasma membrane and FM4-64 stained vesicles. Conclusions/Significance: Our findings indicate that LAZ1 functions as a regulator or effector of plant PCD associated with the HR, in addition to its role in acd11-related death. Furthermore, the similar topology of a plant and human DUF300 proteins suggests similar functions in PCD across the eukaryotic kingdoms, although a direct role for TMEM34 in cell death control remains to be established. Finally, the subcellular localization pattern of LAZ1 suggests that it may have transport functions for yet unknown, death-related signaling molecules at the plasma membrane and/or endosomal compartments. In summary, our results validate the utility of the large-scale suppressor screen to identify novel components with functions in plant PCD, which may also have implications for deciphering cell death mechanisms in other organisms

Malinovsky, F.G.

Brodersen, P.

Fiil, B.K.

McKinney, L.V.

Thorgrimsen, S.

Beck, M.

Nielsen, Henrik Bjørn

Pietra, S.

Zipfel, C.

Robatzek, S.

Petersen, M.

Hofius, D.

Mundy, J.

Online Research Database In Technology

Lazarus1, a DUF300 Protein, Contributes to Programmed Cell Death Associated with Arabidopsis acd11 and the Hypersensitive Response

Malinovsky, Frederikke G.

Nielsen, H. Bjørn

University of East Anglia digital repository

https://curis.ku.dk/portal/files/44032248/Lazarus1.pdf

Lazarus1, a DUF300 Protein, Contributes to Programmed Cell Death Associated with Arabidopsis acd11 and the Hypersensitive Response

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