Author Institution: Department of Biological Sciences, Kent State University, Kent, Ohio 4424

Brockett, Bruce L.

The Ohio Journal of Science

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CHROMOSOME NUMBER OF SIUM SUAVE.1—Conflicting chromosomecounts for Sium suave Walt, occur in the literature. Bell and Constance (1960)report chromosome counts of n = 6 for plants from Illinois, South Carolina, andOregon. Liu (1961) records a chromosome number of n = 6 for plants from Taiwan.The only mitotic count reported is by Mulligan (1961), who reports a chromosomenumber of 2n = 22 for plants from Ottawa, Canada.In an effort to end this discrepancy, mitotic counts were made on plants fromAshtabula County, Ohio. In addition, the herbarium specimens from whichMulligan made his counts were examined. As a result of this examination andby using keys in manuals, Mulligan's plants were determined to be Cicuta bulbiferaL., despite the following label information on the sheet. "Sium suave Walt.Ottawa, edge of Rideau Canal. Chromosome count of 2w = 22 determined onroot tips from these two plants. G. A. Mulligan #2463. Aug. 6, 1959". Be-cause of this error in identification, this count must be discarded as a chromosomenumber for Sium suave. Appreciation is expressed to W. J. Cody, Curator,Phanerogamic Herbarium, Plant Research Institute, Department of Agriculture,Ottawa, Canada, for the loan of the above specimen.FIGURE 1. Chromosomes in root tip cells of Sium suave. A. Photomicrograph of metaphase.X1800. B & C. Photomicrograph and camera lucida drawing of anaphase.X1400.Determination of the mitotic count on plants of Sium suave from AshtabulaCounty, Ohio, was made as follows. Root tips were fixed in a mixture of 6 partsabsolute methanol : 3 parts chloroform : 2 parts propionic acid, and stained in 2%lactic-acetic-orcein (Cooperrider and Morrison, 1967). A chromosome number of2n = 12 (Figure 1) was found in 25 cells from two plants. Voucher specimenswere deposited in the Kent State University Herbarium; the collection data aregiven below.Ohio. Ashtabula County: swamp in Public Hunting Area two miles southeastof New Lyme, Brockett #575, 587.—BRUCE L. BROCKETT; Department ofBiological Sciences, Kent State University, Kent, Ohio 44240.Manuscript received August 3, 1968.LITERATURE CITEDBell, C. R. and L. Constance. 1960. Chromosome numbers in Umbelliferae. II. Am. Jour.Bot. 47: 24-32.Cooperrider, T. S. and J. H. Morrison. 1967. Lactic-acetic-orcein as a chromosome stain.Mich. Bot. 6: 176-178.Liu, T. S. et al. 1961. Umbelliferae of Taiwan. Quart. Jour. Taiwan Mus. 14: 15-47.Mulligan, G. A. 1961. Chromosome numbers of Canadian weeds. III. Canad. Jour. Bot.39: 1057-1066.THE OHIO JOURNAL OF SCIENCE 70(2): 122, March, 1970.

Chromosome Number of Sium Suave

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Chromosome Number of Sium Suave

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