Mycobacteriummarseillense was recently described as a new species belonging to the Mycobacterium avium complex (MAC).We describe a case of pulmonary disease caused by M. marseillense in an immunocompetent patient. All strains isolated from the patient were preliminarily identified as M. intracellulare; however, a retrospective molecular analysis corrected the identification to M. marseillense

Antenora, Federico

Apice, Mariagrazia

Fabbri, Leonardo M.

Fabio, Anna

Gennari, William

Grottola, Antonella

Magnani, Rita

Pecorari, Monica

Roversi, Pietro

Rumpianesi, Fabio

Serpini, Giulia Fregni

Tagliazucchi, Sara

Emerging Infectious Diseases

English

PubMed

Archivio istituzionale della ricerca - Università di Modena e Reggio Emilia

LETTERS  3. Socolovschi C, Mediannikov O, Sokhna C, Tall A, Diatta G, Bassene H, et al. Rick-ettsia felis–associated uneruptive fever, Senegal. Emerg Infect Dis. 2010;16:1140–2. http://dx.doi.org/10.3201/eid1607.100070  4. Yu XJ, Walker DH. Genus Rickettsia da Rocha-Lima 1916. In: Brenner DJ, Krieg NR, Staley JR, editors. Bergey’s manual of systematic bacteriology. Vol. 2, part C. 2nd ed. New York: Springer; 2005, p, 96–106.  5. Leitner M, Yitzhaki S, Rzotkiewicz S, Keysary A. Polymerase chain reaction–based diagnosis of Mediterranean spotted fever in serum and tissue samples. Am J Trop Med Hyg. 2002;67:166–9.  6. Santos FCP, Brasil RA, Nascimento EMM, Angerami RN, Colombo S, Pinter A, et al. Evaluacion de la PCR en tiempo real para el diagnostico de la Fiebre Man-chada Brasilena. Acta Med Costarric. 2013;55(supp):77.  7. Merhej V, Raoult D. Rickettsial evolution in the light of comparative genomics. Biol Rev Camb Philos Soc. 2011;86:379–405. http://dx.doi.org/10.1111/j.1469-185X. 2010.00151.x  8. Merhej V, Georgiades K, Raoult D. Post-genomic analysis of bacterial pathogens repertoire reveals genome reduction rather than virulence factors. Brief Funct Genomics. 2013;12:291–304. http://dx. doi.org/10.1093/bfgp/elt015  9. Behar A, McCormick LJ, Perlman SJ. Rickettsia felis infection in a common household insect pest, Liposcelis bostry-chophila (Psocoptera: Liposcelidae). Appl Environ Microbiol. 2010;76:2280–5. http://dx.doi.org/10.1128/AEM.00026-1010. Thepparit C, Sunyakumthorn P, Guillotte ML, Popov VL, Foil LD, Ma-caluso KR. Isolation of a rickettsial patho-gen from a non-hematophagous arthro-pod. PLoS ONE. 2011;6:e16396. http://dx.doi.org/10.1371/journal.pone.0016396Address for correspondence: David H. Walker, University of Texas Medical Branch—Galveston, 301 University Blvd, Galveston, TX 77555-0609, USA; email: dwalker@utmb.eduPulmonary Disease Caused by  Mycobacterium marseillense, ItalyTo the Editor: Mycobacterium marseillense was recently described as a new species belonging to the Myco-bacterium avium complex (MAC) (1). We describe a case of pulmonary dis-ease caused by M. marseillense in an immunocompetent patient. All strains isolated from the patient were prelimi-narily identified as M. intracellulare; however, a retrospective molecular analysis corrected the identification to M. marseillense.In December 2005, a 65-year-old man was admitted to the Univer-sity Hospital, Modena, Italy, with a 2-week history of fever, cough, and hemoptysis. Physical examination detected diffuse rales, and chest ra-diographs showed a diffuse nodular opacity and bronchial thickening, confirmed by high-resolution com-puted tomography (CT) of the chest (Figure, panel A). The patient had ex-perienced several previous episodes of hemoptysis and persistent produc-tive cough since 1998, and tubular bronchiectasis had been detected on previous high-resolution CT images. The patient had a history of thalas-semia minor, was HIV negative, and was formerly a mild smoker (10 cigarettes/day for 4 years during his youth). He had no chronic disorders and no history of immunosuppres-sive-drug or alcohol use.Bacterial and fungal cultures and a smear for acid-fast bacilli performed on a bronchoalveolar lavage (BAL) sample were all negative. A nontu-berculous mycobacterium strain was isolated by culture and preliminar-ily identified as M. intracellulare by using the GenoType Mycobacterium CM/AS Kit (Hain Lifesciences, Ne-hren, Germany). At that time, a drug susceptibility test for isoniazid, ri-fampin, streptomycin, and ethambutol was improperly performed (i.e., was not applicable for MAC) by using the agar proportion method; sensitiv-ity information for macrolides was unavailable. The strain was resistant to ethambutol and susceptible to the other drugs. The physician prescribed rifampin, isoniazid, and amikacin. After remission of fever and hemop-tysis and improvement of chronic cough, the patient was discharged from the hospital.In March 2006, he was readmit-ted to the hospital for worsening of his condition and onset of side effects associated with rifampin and isonia-zid use. The treatment was discon-tinued and replaced by levofloxacin, terizidone, and azithromycin, which resulted in remission of symptoms. This therapy was continued after hos-pital discharge.In 2007, the patient was twice ad-mitted for follow-up and microbiolog-ical testing to determine bacteriologic status. All 3 separate sputum samples were negative for mycobacteria, other bacteria, and fungi. However, BAL sample culture results were positive for the same mycobacterium despite continued therapy with levofloxacin, terizidone, and azithromycin.During 2008, as an investigation of the possibility of persistent excre-tion of organisms, additional samples were collected 5 times. The sputum cultures were intermittently positive, while the BAL sample cultures were persistently positive.In May 2009, after the patient had been persistently stable and had negative culture results for 14 months, the antimicrobial drug therapy was stopped. In December 2010, the pa-tient’s only symptom was persistent productive cough; however, the spu-tum culture was again positive, and high-resolution CT revealed a wors-ening condition of his lungs (Figure, panel B). A new antimycobacterial drug regimen of ethambutol, rifampin, and azithromycin was started, in accordance with the international 	 Emerging	Infectious	Diseases	•	www.cdc.gov/eid	•	Vol.	20,	No.	10,	October	2014	 1769LETTERS1770	 Emerging	Infectious	Diseases	•	www.cdc.gov/eid	•	Vol.	20,	No.	10,	October	2014guidelines of the American Thoracic Society and the Infectious Diseases Society of America (2).After the patient had received 6 months of therapy, the sputum culture result was again negative. The acid-fast bacilli smear and culture results remained negative until November 2012. The latest treatment resulted in recovery from symptoms and a more stable condition. However, cough and sputum production, although attenu-ated, persisted despite treatment and negative microbiological test results. High-resolution CT images (Figure, panel C) indicated overall progression of pulmonary involvement from the time of first admission.In February 2011, on the basis of the known cross-reactivity of the M. intracellulare probe with most MAC species when the GenoType Myco-bacterium CM/AS assay is used (3), we determined the sequences of a portion of the rpoB gene and inter-nal transcribed spacer–1 region in 2 strains isolated from sputum in March 2006 and June 2010. All sequences overlapped with M. marseillense type strain sequences in GenBank, show-ing an identity of 100% in internal transcribed spacer–1 with EU266631 and 99.8% (1 mismatch) in rpoB with EF584434. We therefore show the association of M. marseillense infection with pul-monary disease in an immunocompetent patient, helping define the clinical fea-tures and natural history of pulmonary disease caused by M. marseillense. We cannot assert whether the clinical course is associated with the intrinsic charac-teristics of M. marseillense infection or with the therapeutic regimen, possibly influenced by numerous adverse effects that may have compromised its effec-tiveness. More careful management in accordance with the American Thoracic Society and the Infectious Diseases So-ciety of America guidelines for manage-ment of nontuberculous mycobacterial diseases could have achieved a more effective course of treatment. More case reports of pulmonary disease caused by M. marseillense are needed to support our observations and to provide more insight into its clinical picture.AcknowledgmentWe are grateful to Alice Artioli for linguistic revision of the manuscript.Antonella Grottola,  Pietro Roversi, Anna Fabio, Federico Antenora,  Mariagrazia Apice,  Sara Tagliazucchi,  William Gennari,  Giulia Fregni Serpini,  Fabio Rumpianesi,  Leonardo M. Fabbri,  Rita Magnani,  and Monica PecorariAuthor	 affiliations:	 University	 Hospital- Policlinico	 and	 University	 of	 Modena	 and	Reggio	Emilia,	Modena,	ItalyDOI:	http://dx.doi.org/10.3201/eid2010.140309References  1. Ben Salah I, Cayrou C, Raoult D, Drancourt M. Mycobacterium marseil-lense sp. nov., Mycobacterium timonense sp. nov. and Mycobacterium bouched-urhonense sp. nov., members of the Myco-bacterium avium complex. Int J Syst Evol Microbiol. 2009;59:2803–8. http://dx.doi.org/10.1099/ijs.0.010637-0  2. Griffith DE, Aksamit T, Brown-Elliott BA, Catanzaro A, Daley C, Gordin F, et al. An official ATS/IDSA statement: diagnosis, treatment, and prevention of nontubercu-lous mycobacterial diseases. Am J Respir Crit Care Med. 2007;175:367–416. http://dx.doi.org/10.1164/rccm.200604-571ST  3. Tortoli E, Pecorari M, Fabio G, Messinò M, Fabio A. Commercial DNA probes for mycobacteria incorrectly identify a num-ber of less frequently encountered species. J Clin Microbiol. 2010;48:307–10. http://dx.doi.org/10.1128/JCM.01536-09Address for correspondence: Antonella Grottola, University Hospital-Policlinico, Via del Pozzo71, 41124 Modena, Italy; email: antonella.grottola@unimore.itFigure.	High-resolution	 computed	 tomographic	 chest	 images	of	 a	man	with	prolonged	pulmonary	disease	 caused	by	Mycobacterium marseillense, Italy.	A)	October	6,	2005.	Bilateral	bronchiectasis,	mainly	in	the	middle	lobe	and	lingula,	associated	with	multiple	nodules	and	middle	 lobe	consolidation.	B)	June	7,	2010.	 Increased	micronodular	opacities,	mainly	 in	 the	 right	middle	and	 lower	 lobes,	and	a	worsening	of	the	bronchiectasis.	C)	August	3,	2013.	Persistence	of	nodular	component,	cavitation,	and	wider	bronchiectasis	with	bronchial	wall	thickening.

Pulmonary disease caused by Mycobacterium marseillense, Italy

Abstract

Similar works

Full text

Available Versions

Archivio istituzionale della ricerca - Università di Modena e Reggio Emilia

Directory of Open Access Journals