Arg-Thz is a minimal substrate for the N-alpha,N-alpha-arginyl methyltransferase involved in the biosynthesis of plantazolicin

Abstract

Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich.This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.The final biosynthetic step towards plantazolicin (PZN) comprises Nα,Nα-arginyl methyltransferase (PznL) mediated N-terminal bismethylation. We show that PznL processes truncated desmethyl-plantazolicin analogues, but only those with an N-terminal guanidine side chain derived from arginine. PznL specificity, which is narrow, depends on the side chain of the N-terminal amino acid linked to an azole, and not so much on the number of azoles.DFG, EXC 314, Unifying Concepts in Catalysi