In this study the interactions of Riboflavin (Vitamin B2) with a mercury surface are investigated. Firstly, by using Cyclic
Voltammetry, it is demonstrated that Riboflavin can be efficiently accumulated, by adsorption from buffered solutions
containing an excess of NaClO4, onto the mercury drop of a HMDE. Secondly, it is shown that the adsorbed Riboflavin
can be reduced through an electrochemical reaction whose stoichiometry is extricated by confronting simulated with
experimental CV voltammograms acquired in a range of pH between about four and nine. Finally, the cathodic current,
sustained by the surface reduction of Riboflavin, is exploited for assaying Riboflavin via Differential Pulse Adsorption
Cathodic Stripping Voltammetry (DP AdCSV) within the frame of the standard additions calibration procedure.
By applying the suggested DP AdCSV procedure with standard voltammetric equipment, typical DP settings and
pre-electrolysis time of about 10 s, a linear response is maintained if Riboflavin concentration in the electrolysed solution
does not exceed about 2 mg/l. On the other side, a limit of detection (expressed as the concentration of Riboflavin in the
electrolysed solution) of 7 μg/l has been achieved with a pre-electrolysis time of 68 s
