Bosworth, LA

Cartmell, SH

Downes, S

McGrouther, DA

Wong, J

English

The University of Manchester - Institutional Repository

The University of Manchester ResearchUsing 3D Electrospun Nanofibrous Scaffolds to RepairTendonsLink to publication record in Manchester Research ExplorerCitation for published version (APA):Bosworth, LA., Wong, J., Cartmell, SH., McGrouther, DA., & Downes, S. (2012). Using 3D ElectrospunNanofibrous Scaffolds to Repair Tendons. Poster session presented at Orthopaedic Research Society (ORS),Moscone Convention Center, San Francisco.Citing this paperPlease note that where the full-text provided on Manchester Research Explorer is the Author Accepted Manuscriptor Proof version this may differ from the final Published version. If citing, it is advised that you check and use thepublisher's definitive version.General rightsCopyright and moral rights for the publications made accessible in the Research Explorer are retained by theauthors and/or other copyright owners and it is a condition of accessing publications that users recognise andabide by the legal requirements associated with these rights.Takedown policyIf you believe that this document breaches copyright please refer to the University of Manchester’s TakedownProcedures [http://man.ac.uk/04Y6Bo] or contact uml.scholarlycommunications@manchester.ac.uk providingrelevant details, so we can investigate your claim.Download date:12. Nov. 20223D electrospun nanofibrous scaffolds mimic tendon tissue, confer cell contact guidance, are relatively strong and are biocompatible in vivo. Discussion Controlled orientation and manipulation of fibres has allowed 3D scaffolds, which mimic the tendon ultrastructure to be fabricated.  3D nanofibrous scaffolds yielded tensile strengths comparable to human Achilles tendons1 (36.5 MPa - not tested to rupture).  3D scaffolds were biocompatible and conferred contact guidance to seeded tenocytes.  Scaffolds were successfully implanted in vivo, however, a marked inflammatory reaction was observed.  Cells were visible within the scaffold core, suggesting infiltration.  Following 42 days implantation, the scaffolds appeared to have degraded, which was unexpected for this slow degrading polymer2.    Using 3D Electrospun Nanofibrous Scaffolds to Repair Tendons LA, Bosworth1; J, Wong2; SH, Cartmell1; DA, McGrouther2; S, Downes1 1School of Materials, The University of Manchester, Manchester, UK; 2School of Medicine, The University of Manchester, Manchester, UK Lucy.bosworth@manchester.ac.uk  We propose 3D electrospun nanofibrous scaffolds, which replicate the tendon tissue ultrastructure, as an innovative tendon repair device. SCAFFOLD FABRICATION & PROPERTIES Mechanical	  Property	   3D	  Nanofibrous	  Scaffold	  Young’s	  Modulus	  (MPa)	   68.14	  ±	  23.66	  Tensile	  Strength	  (MPa)	   41.54	  ±	  13.61	  Fig. 1: Methodology for producing 3D electrospun poly(ε-caprolactone) (PCL) fibre scaffolds from a 10 %w/v solution of PCL dissolved in hexafluoro-2-propanol (HFIP). Table 1: Tensile properties of 3D scaffolds, strained to failure using Instron 1122, 5 N load cell and 5 mm/min cross-head speed  (n=5). IN VITRO CHARACTERISATION IN VIVO ASSESSMENT Fig. 2: Scanning electron micrographs of 3D fibre scaffolds with seeded tenocytes adhering and elongating paral lel to underlying fibre direction after 24 hours (a,b). Following 14 days culture, the entire scaffold surface is covered by proliferating cells that are orientated parallel to the scaffolds’ longitudinal axes (c,d). F i g .  3 : S u r g i c a l procedure for c r e a t i n g a p u r p o s e -made defect in the flexor digitorum profundus (FPD) tendon of a mouse.  3D scaffold inserted into defect and held in place by surrounding tissues.  Excised tissue was used as autograft tissue in the opposing FPD tendon. Fig. 4: H&E staining highlighting a dense population of inflammatory cells surrounding the PCL scaffold, with some infiltration.  By 42 days, the scaffold appears to have degraded. Arrows INTRODUCTION Biomaterial scaffolds offer an alternative intervention for the  repair of damaged tendons, where suitable donor tissue is scarce.   Using electrospinning we have fabricated 3D poly(ε-caprolactone) nanofibrous scaffolds, which mimic the hierarchical structure of tendons. Equine superficial digital flexor tenocytes were seeded (50,000 cm-2) on ethanol sterilised and media-treated scaffolds, and cultured up to 14 days. A	  B	  C	  D	  indicate position of graft.  Scale bar = 50 µm.  1 Magnusson et al. (2003) Acta Physiol Scand, 177(2):185-195. 2 Bosworth and Downes. (2010) Polym Degrad Stabil, 95(12):2269-2276.    

Using 3D Electrospun Nanofibrous Scaffolds to Repair Tendons

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Using 3D Electrospun Nanofibrous Scaffolds to Repair Tendons

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