Understanding interactions between microparticles and lipid membranes is of
increasing importance, especially for unraveling the influence of microplastics
on our health and environment. Here, we study how a short-ranged adhesive force
between microparticles and model lipid membranes causes membrane-mediated
particle assembly. Using confocal microscopy, we observe the initial particle
attachment to the membrane, then particle wrapping, and in rare cases
spontaneous membrane tubulation. In the attached state, we measure that the
particle mobility decreases by 26%. If multiple particles adhere to the same
vesicle, their initial single-particle state determines their interactions and
subsequent assembly pathways: 1) attached particles only aggregate when small
adhesive vesicles are present in solution, 2) wrapped particles reversibly
attract one another by membrane deformation, and 3) a combination of wrapped
and attached particles form membrane-mediated dimers, which further assemble
into a variety of complex structures. The experimental observation of distinct
assembly pathways induced only by a short ranged membrane-particle adhesion,
shows that a cellular cytoskeleton or other active components are not required
for microparticle aggregation. We suggest that this membrane-mediated
microparticle aggregation is a reason behind reported long retention times of
polymer microparticles in organisms.Comment: 20 pages, 11 figures (including supporting material
