Chaperone driven polymer translocation through Nanopore: spatial distribution and binding energy

Abstract

Chaperones are binding proteins which work as a driving force to bias the biopolymer translocation by binding to it near the pore and preventing its backsliding. Chaperones may have different spatial distribution. Recently we show the importance of their spatial distribution in translocation and how it effects on sequence dependency of the translocation time. Here we focus on homopolymers and exponential distribution. As a result of the exponential distribution of chaperones, energy dependency of the translocation time will changed and one see a minimum in translocation time versus effective energy curve. The same trend can be seen in scaling exponent of time versus polymer length, $\beta$ ($T\sim\beta$). Interestingly in some special cases e.g. chaperones of size $\lambda=6$ and with exponential distribution rate of $\alpha=5$, the minimum reaches even to amount of less than $1$ ($\beta<1$). We explain the possibility of this rare result and base on a theoretical discussion we show that by taking into account the velocity dependency of the translocation on polymer length, one could truly predict the amount of this minimum