Grbović, Leposava

Momčilov, Paja

Radenković, Miroslav

Radunović, Nebojša

BMC Pharmacology

English

PubMed

Miroslav Radenković

Nebojša Radunović

Paja Momčilov

Leposava Grbović

Springer - Publisher Connector

BioMed Central
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BMC Pharmacology
Open AccessMeeting abstract
Characterization of receptors involved in serotonin contractions of 
isolated human umbilical artery in uncomplicated pregnancy
Miroslav Radenković*1, Nebojša Radunović2, Paja Momčilov2 and 
Leposava Grbović1
Address: 1Department of Pharmacology, Clinical Pharmacology and Toxicology, Medical Faculty, University of Belgrade, 11129 Belgrade, Serbia 
and 2Institute of Obstetrics and Gynecology, Medical Faculty, University of Belgrade, 11000 Belgrade, Serbia
Email: Miroslav Radenković* - miroslavr@beotel.net
* Corresponding author    
Background
Serotonin (5-HT), is a vasoactive substance involved in
physiological functioning of fetoplacental blood flow and
continuous modulation of smooth muscle tone in umbil-
ical vein and arteries. Since umbilical blood vessels have
been shown to be deficient in autonomic innervation, the
action of local autocrine vasoactive substances (including
serotonin) is of considerable importance. Although seven
main groups of 5-HT receptors (5-HT1-7) have been char-
acterized, it has been established that only several 5-HT
receptor subtypes may be involved in vascular effects of
this biogenic amine. Therefore, the aim of this study was
to characterize receptors involved in serotonin-induced
effect on isolated human umbilical artery (HUA) in
uncomplicated pregnancy.
Methods
The experiments were performed on intact vascular rings
of HUA isolated from umbilical cords that were obtained
immediately after vaginal delivery in women with uncom-
plicated pregnancy. Only the remnant tissue, which
would have been otherwise disposed of, has been utilized.
Isometric tension of suspended artery rings was continu-
ously recorded. Contraction induced by each concentra-
tion of 5-HT was later expressed as a percentage of the
maximal contraction induced by Krebs bicarbonate solu-
tion with 60 mM KCl.
Results
5-HT (1 nM - 30 μM) produced concentration-dependent
contractions of HUA. Control contractions produced by
5-HT were notably reduced by methiothepin (a nonselec-
tive 5-HT1/5-HT2 receptor antagonist; 0.01-1 μM), with
typical irreversible competitive antagonism exposed. On
the other hand, increasing concentrations of ketanserin (a
selective 5-HT2A receptor antagonist; 0.03-0.3 μM) signifi-
cantly shifted 5-HT control curves to the right in a concen-
tration-dependent manner with reversible competitive
antagonism shown.
Conclusion
Schild's analysis of the effect produced by ketanserin,
taken together with the results obtained with methi-
otepin, suggest that the transduction mechanism of HUA
responses to serotonin in uncomplicated pregnancy
involves activation of a mixed population of 5-HT1 and 5-
HT2A receptors.
Acknowledgements
This research was supported by grant 145015B from the Ministry of Sci-
ence, Serbia.
from 15th Scientific Symposium of the Austrian Pharmacological Society (APHAR) Joint meeting with the Hungarian Society of Experimental and Clinical 
Pharmacology (MFT) and the Slovenian Pharmacological Society (SDF)
Graz, Austria. 19-21 November 2009
Published: 12 November 2009
BMC Pharmacology 2009, 9(Suppl 2):A14 doi:10.1186/1471-2210-9-S2-A14
<supplement> <title> <p>15th Scientific Symposium of the Austrian Pharmacological Society (APHAR)</p> </title> <editor>Andrea Laslop and Thomas Griesbacher</editor> <note>Meeting abstracts - A single PDF containing all abstracts in this Supplement is available <a href="http://www.biomedcentral.com/content/files/pdf/1471-2210-9-S2-full.pdf">here</a>.</note> <url>http://www.biomedcentral.com/content/pdf/1471-2210-9-S2-info.pdf</url> </supplement>
This abstract is available from: http://www.biomedcentral.com/1471-2210/9/S2/A14
© 2009 Radenković et al; licensee BioMed Central Ltd. 


Characterization of receptors involved in serotonin contractions of isolated human umbilical artery in uncomplicated pregnancy

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Characterization of receptors involved in serotonin contractions of isolated human umbilical artery in uncomplicated pregnancy

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