Biosurfactant compounds are produced by microorganisms. These isolates reduced surface tension both in aqueous solutions and hydrocarbon mixtures. The objective of this study was to isolate and characterize the surface active components from the crude biosurfactant produced by Pseudomonas aeruginosa PAVIJ from contaminated soil. This organism was grown on four different carbon sources (palm oil, coconut oil, honge oil and castor oil). To confirm the ability of isolates in biosurfactant production, different screening methods including blood hemolysis, emulsification, bacterial adherence test for hydrocarbon (BATH) assay, determination of surface tension, drop-collapse, cetyl tri ammonium bromide (CTAB) and methylene blue reduction assay were assessed. The fraction rich in glycolipids was obtained by the fractionation of crude biosurfactant using solid phase extraction and further indentified using thin layer chromatography and confirmed through High performance liquid chromatography. Moreover, this glycolipid rich fractions was found to be antimicrobial agent against several bacterial strains isolated from clinical samples of infected patients. Further purification steps should be carefully analyzed as each purification step will increase the costs and decreases the amounts of biosurfactants recovered
