AIM: To determine the association of Helicobacter pylori (H pylori) CagA+ infection and pro-inflammatory polymorphisms of the genes interleukin (IL)-1RN and IL-1B with the risk of gastric atrophy and peptic ulcers in a dyspeptic population in Costa Rica, a country with high incidence and mortality of gastric cancer

Alpízar-Alpízar, Warner

Cuenca, Patricia

de Mascarel, Antoine

González, María I

Mégraud, Francis

Pérez-Pérez, Guillermo

Ramírez, José A

Ramírez, Vanessa

Sierra, Rafaela

Une, Clas

PubMed

Rafaela Sierra

Clas Une

Vanessa Ramírez

Warner Alpízar-Alpízar

María I González

José A Ramírez

Antoine de Mascarel

Patricia Cuenca

Guillermo Pérez-Pérez

Francis Mégraud

Crossref

Relation of atrophic gastritis with Helicobacter pylori -CagA+ and interleukin-1 gene polymorphisms

Artículo científico -- Universidad de Costa Rica. Instituto de Investigaciones en Salud, 2008AIM: To determine the association of Helicobacter pylori (H pylori) CagA+ infection and pro-inflammatory polymorphisms of the genes interleukin (IL)-1RN and IL-1B with the risk of gastric atrophy and peptic ulcers in a dyspeptic population in Costa Rica, a country with high incidence and mortality of gastric cancer.

METHODS: Seven biopsy specimens, a fasting blood sample and a questionnaire concerning nutritional and sociodemographic factors were obtained from 501 consecutive patients who had undergone endoscopy for dyspeptic symptoms. A histopathological diagnosis was made. Pepsinogen concentrations were analyzed by enzyme linked immunosorbent assay (ELISA). Infection with H pylori CagA+ was determined by serology and polymerase chain reaction (PCR). IL-1B and IL-1RN polymorphisms genotyping was performed by PCR-restriction fragment length polymorphism (PCR-RFLP) and PCR respectively.

RESULTS: In this dyspeptic population, 86% were H pylori positive and of these, 67.8% were positive for CagA. Atrophic antral gastritis (AAG) was associated with CagA+ status [odd ratio (OR) = 4.1; P < 0.000] and fruit consumption (OR = 0.3; P < 0.00). Atrophic body gastritis (ABG) was associated with pepsinogen PGI/PGII < 3.4 (OR = 4.9; P < 0.04) and alcohol consumption (OR = 7.3; P < 0.02). Duodenal ulcer was associated with CagA+ (OR = 2.9; P < 0.04) and smoking (OR = 2.4; P < 0.04). PGI < 60 μg/L as well as PGI/PGII < 3.4 were associated with CagA+.

CONCLUSION: In a dyspeptic population in Costa Rica, H pylori CagA+ is not associated with ABG, but it is a risk factor for AAG. The pro-inflammatory cytokine polymorphisms IL-1B + 3945 and IL-1RN are not associated with the atrophic lesions of this dyspeptic population.Universidad de Costa Rica. Instituto de Investigaciones en SaludUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto de Investigaciones en Salud (INISA

Sierra Ramos, Rafaela

Une, Clas Allan

Ramírez Mayorga, Vanessa

Alpízar Alpízar, Warner

González, María I.

Ramírez, José A.

Cuenca Berger, Patricia

Pérez Pérez, Guillermo Ignacio

Repositorio Institucional de la Universidad de Costa Rica

World Journal of Gastroenterology

Relation of atrophic gastritis with Helicobacter pylori-CagA+ and interleukin-1 polymorphisms

http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2773333

Relation of atrophic gastritis with Helicobacter pylori-CagA+ and interleukin-1 gene polymorphisms

Relation of atrophic gastritis with Helicobacter pylori-CagA+ and interleukin-1 gene polymorphisms

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Repositorio Institucional de la Universidad de Costa Rica