Providing biocatalysts through customizing lipases by different processes

Abstract

The author is recalling first mechanisms of lipase catalyzed reactions. Hydrolyses and syntheses of triglycerides and esters, interesterification: generally these types of reactions are designed under the generic designation of acyl transfers. Enzymatic reactions are equilibrated, and then the displacement of the reaction in one or the other direction is highly depending on the thermodynamic activity of water (aw). This notion is discussed in depth. It is also emphasized on the quantity and quality of interface and also on the concept of pH memory or ionic force memory of the aqueous phase even whether this lost is confined or invisible. Lipases selectivity is especially developed and the author emphasizes on the possibility of changing selectivities through different perturbations. Physical and chemical modifications of lipases are then described throught the more pedagogic and spectacular examples. The author is also stressing on the mastership of reactions and processes imposing a task to lipases. He is finally concluding that in the space of fifty years, one ascertains a colassol work has been accomplished and that henceforward very current reactions for commodity products are available as certain companies are already doing. He is pointing out plant lipases are on the verge to meet deep and unexpected developments in a very near future since their production through agriculture is probably the lower cost mean of production. (Résumé d'auteur