Bratt, G

Broliden, K

Hasselrot, K

Hirbod, T

Sandström, E

Säberg, P

English

PubMed

Springer - Publisher Connector

BioMed Central
Page 1 of 1
(page number not for citation purposes)
Retrovirology
Open AccessPoster presentation
P04-16. Orally exposed uninfected individuals have systemic 
anti-HIV response correlating with partner's viral load
K Hasselrot*, G Bratt, T Hirbod, P Säberg, E Sandström and K Broliden
Address: Medicine, Karolinska Institute, Stockholm, Sweden
* Corresponding author    
Background
We have previously shown that this cohort of exposed
uninfected individuals (EU) of men who have sex with
men (MSM) display HIV-1 neutralizing capacity in their
salivary IgA1 (AIDS 2009). To investigate whether oral
HIV-1 exposure mounts also a systemic anti-HIV-1
response, we have here investigated consecutive samples
of plasma (unfractioned and IgA1 purified) for neutraliz-
ing capacity, and whether the response correlates with
exposure measured by viral load in the HIV-positive part-
ners.
Methods
Plasma were collected from EU (n = 25) every 6 months
during 2 years and from low-risk controls (n = 22). IgA1-
purification was performed with jacaline. Neutralization
was tested in a PBMC-based neutralization assay using
three primary HIV-1 isolates. Self-reported questionnaires
described routes of HIV-1-exposure, and clinical records
determined viral load in HIV-positive partners.
Results
At study start, plasma samples from 7/25 EU could neu-
tralize 2 of 3 tested HIV-1 isolates. None of the 22 low-risk
controls could neutralize any HIV-1 isolate (p = 0.01). 6/
7 EU retained their neutralization capacity during contin-
uous exposure, although 3/6 EU only neutralized one of
the three HIV-1 isolates at time of last sampling. Of
importance, the neutralization capacity among EU corre-
lated with a significantly higher viral load in the respective
partners compared to partners of non-neutralizing EU.
Purified IgA1 was shown to mediate neutralization in 6/7
samples whereas none of the IgA1-depleted plasma sam-
ples could neutralize HIV-1.
Conclusion
Orally exposed uninfected MSM can mount an anti-HIV-
1 response in plasma, here shown to correlate with the
viral load in the HIV-positive partner.
from AIDS Vaccine 2009
Paris, France. 19–22 October 2009
Published: 22 October 2009
Retrovirology 2009, 6(Suppl 3):P44 doi:10.1186/1742-4690-6-S3-P44
<supplement> <title> <p>AIDS Vaccine 2009</p> </title> <editor>Anna Laura Ross</editor> <note>Meeting abstracts – A single PDF containing all abstracts in this Supplement is available <a href="http://www.biomedcentral.com/content/files/pdf/1742-4690-6-S3-full.pdf">here</a>.</note> <url>http://www.biomedcentral.com/content/pdf/1471-2105-10-S12-info.pdf</url> </supplement>
This abstract is available from: http://www.retrovirology.com/content/6/S3/P44
© 2009 Hasselrot et al; licensee BioMed Central Ltd. 


P04-16. Orally exposed uninfected individuals have systemic anti-HIV response correlating with partner's viral load

K Hasselrot

G Bratt

T Hirbod

P Säberg

E Sandström

K Broliden

file:///data/remote/core/dit/data/Springer-OA/pdf/d4f/aHR0cDovL2xpbmsuc3ByaW5nZXIuY29tLzEwLjExODYvMTc0Mi00NjkwLTYtUzMtUDQ0LnBkZg==.pdf

P04-16. Orally exposed uninfected individuals have systemic anti-HIV response correlating with partner's viral load

Abstract

Similar works

Full text

Available Versions

Springer - Publisher Connector

Springer - Publisher Connector