Banchereau, J

Cobb, A

Jutras, D

King, B

Levy, Y

Loof, N

Montes, M

Plants, J

Quinn, C

Sloan, L

Zurawski, S

Retrovirology

English

PubMed

M Montes

N Loof

A Cobb

D Jutras

C Quinn

J Plants

S Zurawski

B King

L Sloan

Y Levy

J Banchereau

Springer - Publisher Connector

P16-49. Broad types of cytokines secreted by Gag-specific T cells from HIV infected patients on HAART

Crossref

BioMed Central
Page 1 of 1
(page number not for citation purposes)
Retrovirology
Open AccessPoster presentation
P16-49. Broad types of cytokines secreted by Gag-specific T cells 
from HIV infected patients on HAART
M Montes1, N Loof1, A Cobb1, D Jutras1, C Quinn1, J Plants1, S Zurawski1, 
B King1, L Sloan1, Y Levy2 and J Banchereau*1
Address: 1HIV Vaccine, Baylor Institute for Immunology Research, Dallas, TX, USA and 2INSERM U841, Créteil, France
* Corresponding author    
Background
Previously described highly immunogenic epitopes from
Gag are able to stimulate Th1/CTL cells. This response is
essential for controlling progression of HIV infection.
Techniques like IFN-γ-ELISPOT and IFN-γ intracellular
staining are currently the standard methods to define anti-
gen-specific immune responses. A recent method, the
Luminex analysis allows the identification of responses
characteristic of T cell subtypes through the secretion of
not only IFN-γ but other key cytokines (IL-5, IL-13, IL-10,
IL-21 and IL-17).
Methods
We have focused on defining the appropriate conditions
to study antigen-specific T cell memory responses in
chronically infected HIV patients. Briefly, short-term
PBMC cultures with 15-mer overlapping peptides from
Gag in the presence of IL-2 favor expansion of antigen-
specific T cells. Luminex analysis of the culture superna-
tants, permits simultaneous identification of cytokine
profiles. HIV-specific clones obtained with selected Gag
peptides were stimulated with different concentrations of
antigens followed by cytokine secretion analysis.
Results
We identified anti-Gag responses to already well known
peptides as well as new ones consistent with each patient's
HLA profile. Interestingly, this analysis revealed that some
epitopes induced a complex pattern of cytokine secretion,
including Th1/Th17, Th2 and IL-21 responses. CD4+ T
cell clones expressing IL-21 are able to secrete Th1 and
Th2 cytokines as well. The same results were obtained
with CD8+ clones; however the secretion of IL-21 is lower
than in CD4+ cells. In the CD4+ and CD8+ T cell clones
the responses are directed to Th1 or Th2 based on the con-
centration of the antigen used to stimulate the cells. How-
ever, Th17 specific cells were only able to secrete IL-17 in
an antigen-specific test.
Conclusion
Our approach will help monitor therapeutic vaccine
responses in planned clinical trials by establishing knowl-
edge of each patient's immune status, thereby permitting
comparison between correlates of viral replication control
and vaccine-elicited immune responses.
from AIDS Vaccine 2009
Paris, France. 19–22 October 2009
Published: 22 October 2009
Retrovirology 2009, 6(Suppl 3):P278 doi:10.1186/1742-4690-6-S3-P278
<supplement> <title> <p>AIDS Vaccine 2009</p> </title> <editor>Anna Laura Ross</editor> <note>Meeting abstracts – A single PDF containing all abstracts in this Supplement is available <a href="http://www.biomedcentral.com/content/files/pdf/1742-4690-6-S3-full.pdf">here</a>.</note> <url>http://www.biomedcentral.com/content/pdf/1471-2105-10-S12-info.pdf</url> </supplement>
This abstract is available from: http://www.retrovirology.com/content/6/S3/P278
© 2009 Montes et al; licensee BioMed Central Ltd. 


Montes, Monica

Loof, Nicolas

Cobb, Amanda

Jutras, David

Quinn, Charlie

Zurawski, Sandra

King, Bryan

Sloan, Louis

Levy, Yves

Banchereau, Jacques

HAL-Inserm

HAL - UPEC / UPEM

HAL: Hyper Article en Ligne

https://inserm.hal.science/inserm-00663921v1/file/1742-4690-6-S3-P278.pdf

P16-49. Broad types of cytokines secreted by Gag-specific T cells from HIV infected patients on HAART

Abstract

Similar works

Full text

Available Versions

Springer - Publisher Connector

Crossref

Crossref

Springer - Publisher Connector

HAL-Inserm

HAL - UPEC / UPEM

HAL: Hyper Article en Ligne