Collier, AC

Deng, WC

Herbeck, JT

Mullins, JI

Rolland, MT

English

PubMed

JT Herbeck

MT Rolland

WC Deng

AC Collier

JI Mullins

Springer - Publisher Connector

P07-06. HIV-1 transmission and early evolution: whole genome analysis

Crossref

BioMed Central
Page 1 of 1
(page number not for citation purposes)
Retrovirology
Open AccessPoster presentation
P07-06. HIV-1 transmission and early evolution: whole genome 
analysis
JT Herbeck, MT Rolland, WC Deng, AC Collier and JI Mullins*
Address: Microbiology, University of Washington, Seattle, WA, USA
* Corresponding author    
Background
Understanding the initial events occuring after transmis-
sion of HIV-1 to a new host is key to determining the
requirements for protective immunity.
Methods
We studied nine individuals in acute HIV-1 infection
(starting in Feibig stages I or II), including four index:sero-
converter partner pairs, with 10 or more viral whole
genome sequences collected at serially sampled visits (up
to 12) for up to one year after infection.
Results
Comparison between index and seroconverter sequences
showed that the dominant HIV-1 variant in the index
partner was not necessarily the founder variant in the
seroconverter. While HIV-1 infection was established by 2
variants in 2 individuals, 7 individuals presented single
founding variants, which showed remarkably homogene-
ous viral population at the earliest time-points. Intra-
patient viral diversity accumulated gradually in all indi-
viduals. Given the rapid population growth that occurs in
acute infection, and the subsequent decline in this popu-
lation at the time of seroconversion, we tested for devia-
tions from neutral evolution and found that the earliest
time-points were more likely to deviate from apparent
neutrality. Cytotoxic T Lymphocyte (CTL) pressure was
manifest over the whole proteome and predominantly
visible in variable proteins, Env and Nef in particular.
Longitudinal analyses showed mutations becoming con-
sensus – as early as day 21 in Tat – and complete displace-
ments of epitopes found at baseline – as early as day 53 in
Env. Overall, we found more evidence of escape muta-
tions than reversions.
Conclusion
We showed that a single viral variant establishes infection
in most successful transmissions and that the dramatic
CD4 T-cell depletion and viral population expansion
observed in acute infection leave signature traces on the
viral sequence population. We also found that the evolu-
tionary response of HIV-1 to CTL pressure is early, com-
plex, and dominated by escape mutations, while
reversions might play a lesser role than previously
thought.
from AIDS Vaccine 2009
Paris, France. 19–22 October 2009
Published: 22 October 2009
Retrovirology 2009, 6(Suppl 3):P104 doi:10.1186/1742-4690-6-S3-P104
<supplement> <title> <p>AIDS Vaccine 2009</p> </title> <editor>Anna Laura Ross</editor> <note>Meeting abstracts – A single PDF containing all abstracts in this Supplement is available <a href="http://www.biomedcentral.com/content/files/pdf/1742-4690-6-S3-full.pdf">here</a>.</note> <url>http://www.biomedcentral.com/content/pdf/1471-2105-10-S12-info.pdf</url> </supplement>
This abstract is available from: http://www.retrovirology.com/content/6/S3/P104
© 2009 Herbeck et al; licensee BioMed Central Ltd. 


Mullins JI

Collier AC

Deng WC

Rolland MT

Herbeck JT

Directory of Open Access Journals

Retrovirology

file:///data/remote/core/dit/data/Springer-OA/pdf/0f7/aHR0cDovL2xpbmsuc3ByaW5nZXIuY29tLzEwLjExODYvMTc0Mi00NjkwLTYtUzMtUDEwNC5wZGY=.pdf

P07-06. HIV-1 transmission and early evolution: whole genome analysis

Abstract

Similar works

Full text

Available Versions

Springer - Publisher Connector

Crossref

Springer - Publisher Connector

Directory of Open Access Journals