Currier, JR

de Souza, MS

Eller, L

Eller, MA

Guwatudde, D

Kijak, GH

Koehler, RN

Marovich, MA

Michael, NL

Robb, ML

Sandberg, JK

Wabwire-Mangen, F

Retrovirology

English

PubMed

MA Eller

L Eller

RN Koehler

GH Kijak

D Guwatudde

MA Marovich

NL Michael

MS de Souza

F Wabwire-Mangen

ML Robb

JR Currier

JK Sandberg

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P10-12. Altered NK cell phenotype and function in Ugandans with chronic HIV-1 infection

de, Souza MS

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Altered NK cell phenotype and function in Ugandans with chronic HIV-1 infection

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(page number not for citation purposes)
Retrovirology
Open AccessPoster presentation
P10-12. Altered NK cell phenotype and function in Ugandans with 
chronic HIV-1 infection
MA Eller1, L Eller1, RN Koehler2, GH Kijak2, D Guwatudde3, MA Marovich2, 
NL Michael2, MS de Souza4, F Wabwire-Mangen3, ML Robb2, JR Currier*2 
and JK Sandberg5
Address: 1Makerere University Walter Reed Project, Kampala, Uganda, 2US Military HIV Research Program (MHRP), Rockville, MD, USA, 
3Makerere University Walter Reed/Makerere Univ School of Public Health, Kampala, Uganda, 4Armed Forces Research Institute of Medical 
Sciences, Bangkok, Thailand and 5Center for Infectious Medicine, Karolinska Institutet, Stockholm, Sweden
* Corresponding author    
Background
NK cells are innate lymphocytes that play a significant role
in the control of viral infections, including HIV-1. We
examined the state of the NK cell compartment in Ugan-
dans with untreated HIV-1 infection in comparison with
matched uninfected controls and determined the associa-
tion of host genetics and inhibitory KIR expression on dis-
ease progression.
Methods
The function and phenotype of NK cells was investigated
using 10-color flow cytometry from frozen PBMC.
Sequence-specific priming (SSP) real-time PCR was used
to genotype for KIR3DL1/DS1 and HLA-B Bw4/Bw6
allowing the discrimination of Bw4 alleles having isoleu-
cine at position 80.
Results
NK cells displayed elevated production of IFN-γ and MIP-
1β, as well as CD107a degranulation in infected subjects.
HIV-1 infection was associated with reduced expression of
KIR2DL1, NKG2A, CD161 and NKp30 in CD56 dim and
CD56neg NK cells, whereas lowered CD161 expression
was the only alteration in the CD56 bright subset. Inter-
estingly, low CD4 counts were associated with increased
levels of IFN-γ and degranulation in CD56 bright NK cells.
The presence of HLA-B Bw4-80I was associated with ele-
vated frequencies of KIR3DL1+ NK cells in both healthy
and chronically HIV-1 infected Ugandans. Furthermore, a
positive correlation was observed between the size of the
KIR3DL1-expressing NK cell subset and viral load only in
Bw4-80I+ patients. Finally, increasing size of this subset
was associated with higher production of MIP-1β, a CC
chemokine with anti-HIV activity, in the NK cell compart-
ment of Bw4-80I+ patients.
Conclusion
NK cells in HIV-1 infected Ugandans display elevated
activity, despite an altered functional and phenotypic pro-
file. Furthermore, specific alterations in the CD56 bright
and CD56 dim subsets occur in patients with severe CD4
loss. In addition, our results suggest that the presence of
Bw4-80I directs an expansion of functional KIR3DL1+ NK
cells, which may be an important source of MIP-1β in
chronic HIV-1 infection.
from AIDS Vaccine 2009
Paris, France. 19–22 October 2009
Published: 22 October 2009
Retrovirology 2009, 6(Suppl 3):P143 doi:10.1186/1742-4690-6-S3-P143
<supplement> <title> <p>AIDS Vaccine 2009</p> </title> <editor>Anna Laura Ross</editor> <note>Meeting abstracts – A single PDF containing all abstracts in this Supplement is available <a href="http://www.biomedcentral.com/content/files/pdf/1742-4690-6-S3-full.pdf">here</a>.</note> <url>http://www.biomedcentral.com/content/pdf/1471-2105-10-S12-info.pdf</url> </supplement>
This abstract is available from: http://www.retrovirology.com/content/6/S3/P143
© 2009 Eller et al; licensee BioMed Central Ltd. 


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P10-12. Altered NK cell phenotype and function in Ugandans with chronic HIV-1 infection

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