Cavarelli, M

Dispinseri, S

Scarlatti, G

Schuitemaker, H

van Nuenen, A

English

PubMed

Scarlatti G

Schuitemaker H

van Nuenen A

Cavarelli M

Dispinseri S

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Retrovirology

P07-04. HIV-1 evolution in mother to child transmission and pediatric disease progression

S Dispinseri

M Cavarelli

A van Nuenen

H Schuitemaker

G Scarlatti

Crossref

BioMed Central
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(page number not for citation purposes)
Retrovirology
Open AccessPoster presentation
P07-04. HIV-1 evolution in mother to child transmission and 
pediatric disease progression
S Dispinseri*1, M Cavarelli1, A van Nuenen2, H Schuitemaker2 and 
G Scarlatti1
Address: 1Viral Evolution and Transmission Unit, San Raffaele Scientific Institute, Milan, Italy and 2Academic Medical Center University, 
Amsterdam, Netherlands
* Corresponding author    
Background
CCR5 using HIV-1 variants are preferentially transmitted
and have an intrinsic phenotypic variability playing an
important role in AIDS pathogenesis. This variation con-
fers a different susceptibility to CC-chemokines as well as
a different capacity to infect ad hoc engineered cell lines
expressing CCR5/CXCR4 chimeric receptors. In this study
we explored the predictive value of R5 variability in
mother-to-child transmission (MTCT) of HIV-1 and pedi-
atric disease progression.
Methods
Virus isolates from 21 transmitting mothers and their chil-
dren obtained close to birth, and 171 biological viral
clones of 5 mother-child pairs were tested for their ability
to infect U87.CD4+ cells expressing wild type receptors
CCR5 or CXCR4, or one of the 6 CCR5/CXCR4 chimeric
receptors, hybrids between CCR5 and CXCR4.
Results
The transmitting mothers carried R5X4 viruses in 5 cases,
R5 broad in 10 and R5 narrow in 11 cases. The detailed
analysis of the chimeric receptor usage showed that the
clones of mothers with R5 broad viruses had a mixture
containing also R5 narrow. To understand if a selective
process occurs during transmission, we compared the
viral phenotype of the mother-child couples. All 11 moth-
ers harbouring an R5 narrow virus transmitted the same
phenotype to their child. Interestingly, the 10 mothers
carrying R5 broad viruses transmitted in all but two case
an R5 broad virus. The children's biological clones
showed that both R5 narrow and R5 broad viruses were
transmitted. The five mothers with an R5X4 transmitted
the whole spectrum of virus phenotypes. The presence of
R5 broad and R5X4 in the children close to birth corre-
lated significantly with severe decline of CD4+ T cells or
death within 2 years.
Conclusion
Our results show that HIV-1 with broad chimeric receptor
usage are not hampered during MTCT and are predictive
of disease progression. This may be of relevance for thera-
peutic intervention, as we showed that R5 broad viruses
are less sensitive to RANTES inhibition.
from AIDS Vaccine 2009
Paris, France. 19–22 October 2009
Published: 22 October 2009
Retrovirology 2009, 6(Suppl 3):P102 doi:10.1186/1742-4690-6-S3-P102
<supplement> <title> <p>AIDS Vaccine 2009</p> </title> <editor>Anna Laura Ross</editor> <note>Meeting abstracts – A single PDF containing all abstracts in this Supplement is available <a href="http://www.biomedcentral.com/content/files/pdf/1742-4690-6-S3-full.pdf">here</a>.</note> <url>http://www.biomedcentral.com/content/pdf/1471-2105-10-S12-info.pdf</url> </supplement>
This abstract is available from: http://www.retrovirology.com/content/6/S3/P102
© 2009 Dispinseri et al; licensee BioMed Central Ltd. 


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