Berthet, J

Cognasse, F

Damien, P

Garraud, O

Hamzeh-cognasse, H

Lucht, F

Pozzetto, B

English

PubMed

F Cognasse

H Hamzeh-cognasse

J Berthet

P Damien

F Lucht

B Pozzetto

O Garraud

Springer - Publisher Connector

BioMed Central
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Retrovirology
Open AccessPoster presentation
P10-09. Altered release of RANTES from human, normal platelets: 
contribution of distinct HIV-1MN gp41 peptides
F Cognasse*, H Hamzeh-cognasse, J Berthet, P Damien, F Lucht, B Pozzetto 
and O Garraud
Address: Immunology, EFS Auvergne-Loire – GIMAP-EA3064, Saint-Etienne, France
* Corresponding author    
Background
Blood platelets link the processes of haemostasis and
inflammation. Platelets can further, sense danger by the
display of e.g. TLR, and our group demonstrated that they
can even discriminate between danger signals in order to
secrete cytokines/chemokines differentially. This study
examines the secretion of normal platelets after exposure
to recombinant HIV-1MN-gp120 or gp41 peptides.
Methods
We used platelets sampled from healthy, HIV-1,2 nega-
tive, normal blood donors. Platelet-Rich Plasma (PRP)
was incubated after addition thrombin receptor agonist
peptide or HIV env glycoprotein. PRP were centrifuged
and platelet free supernatant was stored. Two recom-
binant gp120MN (produced in insect cells using the bac-
ulovirus expression system) and synthetic peptides of
gp41MN, and mAbs to gp41: D50 and 2F5, were all
obtained through the AIDS Research and Reference Rea-
gent Program. Soluble CD62p and RANTES production in
cultures were measured by specific ELISA.
Results
Platelets (n = 10) with various HIV-1MN gp120 or
gp41MN peptides had no significant effect on sCD62P
release. None of two recombinant gp120MN tested
caused any modulation of RANTES release. In contrast,
the stimulation of platelets with gp41 peptides evidenced
that peptides 2025 (GKLICTTTVPWNASWSNKSL) and
2031 (LLELDKWASLWNWFDITNWL) led to a significant
reduction of RANTES release, whereas the other 8 tested
peptides were ineffective. RANTES production could be
significantly restored if platelet cultures with peptides
2025 and 2031 were performed in the concurrent pres-
ence of D50 or 2F5 mAb anti-gp41.
Conclusion
This data indicates that certain peptides of HIV env-gp41
exert a negative signal, the precise nature of which
remains to be ascertained and further investigated, for
RANTES production by normal platelets in in vitro stimu-
lation conditions. Our data provide novel information on
possible primary interactions between platelets and HIV
in their early encounter in the circulation. Therefore, fur-
ther studies are needed to clarify its consequence: fair or –
more likely – foe?
from AIDS Vaccine 2009
Paris, France. 19–22 October 2009
Published: 22 October 2009
Retrovirology 2009, 6(Suppl 3):P140 doi:10.1186/1742-4690-6-S3-P140
<supplement> <title> <p>AIDS Vaccine 2009</p> </title> <editor>Anna Laura Ross</editor> <note>Meeting abstracts – A single PDF containing all abstracts in this Supplement is available <a href="http://www.biomedcentral.com/content/files/pdf/1742-4690-6-S3-full.pdf">here</a>.</note> <url>http://www.biomedcentral.com/content/pdf/1471-2105-10-S12-info.pdf</url> </supplement>
This abstract is available from: http://www.retrovirology.com/content/6/S3/P140
© 2009 Cognasse et al; licensee BioMed Central Ltd. 


P10-09. Altered release of RANTES from human, normal platelets: contribution of distinct HIV-1MN gp41 peptides

Pozzetto B

Lucht F

Damien P

Berthet J

Hamzeh-cognasse H

Cognasse F

Garraud O

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P10-09. Altered release of RANTES from human, normal platelets: contribution of distinct HIV-1MN gp41 peptides

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