Almond, N

Berkhout, B

Cranage, M

Das, AT

Klaver, B

Kleibeuker, W

Manoussaka, MS

Page, M

Quartey-Papafio, R

Robinson, M

Stebbings, R

English

PubMed

MS Manoussaka

R Stebbings

R Quartey-Papafio

M Robinson

M Page

AT Das

W Kleibeuker

B Klaver

B Berkhout

N Almond

M Cranage

Springer - Publisher Connector

BioMed Central
Page 1 of 1
(page number not for citation purposes)
Retrovirology
Open AccessPoster presentation
P11-10. Modulation of intestinal T cells following infection of 
macaques with live attenuated and conditionally 
replication-competent SIV
MS Manoussaka*1, R Stebbings2, R Quartey-Papafio2, M Robinson2, M Page2, 
AT Das3, W Kleibeuker3, B Klaver3, B Berkhout3, N Almond2 and M Cranage1
Address: 1Cellular and Molecular Medicine, St George's University, London, UK, 2National Institute of Biological Standards, Health Protection 
Agency, Potters Bar, UK and 3Academic Medical Centre of the University of Amsterdam, Amsterdam, Netherlands
* Corresponding author    
Background
Live-attenuated SIV can induce superinfection resistance;
however, the mechanism of this effect is not understood
and may have implications for HIV vaccine development.
To further investigate the role of virus replication in con-
ferring protection we have analysed T cell phenotype and
responses in gut tissue following infection of macaques
with either SIVmac239Δnef or a doxycycline-dependent
replication variant of SIVmac239 Δnef designated
SIVrtTA.
Methods
Mononuclear cells (MNC) were recovered from small and
large intestine of four rhesus macaques infected with
SIVrtTA for 26 weeks in the presence of orally-adminis-
tered doxycycline. In two animals doxycycline was with-
drawn for 8 weeks before analysis (Group A) and in 2
macaques analysis was performed at week 26 (Group B).
A further two animals were analysed after 26 weeks of
infection with SIVmac239Δnef (Group C). Peripheral
blood mononuclear cells (PBMC) were recovered at the
same time points. Polychromatic flow cytometry was used
to assess the percentages of central memory (Tcm)
(CD28+CD95+) and effector memory (Tem) (CD28-
CD95+) T cells as well as CD4 and CD8 T responses to SIV
Gag, Rev and Tat by the detection of TNF-α and IL-2.
Results
In animals with actively replicating SIV (Groups B & C)
the percentage of CD4 and CD8 Tcm both in peripheral
blood, and to a lesser extent, in the small and large intes-
tine were relatively low compared to CD4 and CD8 Tem.
In contrast, the reverse pattern was seen in animals where
SIV replication was turned off by withdrawal of doxycy-
cline (Group A). Intestinal MNC responded to Gag pep-
tides producing TNF-α and IL-2. Animals withdrawn from
doxycycline had Rev and Tat-specific CD4+ and CD8+
TNF-α producing T cells in both small and large intestine.
Conclusion
SIVrtTA offers a system for dissecting the parameters of
replication, immune response and protective efficacy to
more fully understand in vivo superinfection resistance.
from AIDS Vaccine 2009
Paris, France. 19–22 October 2009
Published: 22 October 2009
Retrovirology 2009, 6(Suppl 3):P155 doi:10.1186/1742-4690-6-S3-P155
<supplement> <title> <p>AIDS Vaccine 2009</p> </title> <editor>Anna Laura Ross</editor> <note>Meeting abstracts – A single PDF containing all abstracts in this Supplement is available <a href="http://www.biomedcentral.com/content/files/pdf/1742-4690-6-S3-full.pdf">here</a>.</note> <url>http://www.biomedcentral.com/content/pdf/1471-2105-10-S12-info.pdf</url> </supplement>
This abstract is available from: http://www.retrovirology.com/content/6/S3/P155
© 2009 Manoussaka et al; licensee BioMed Central Ltd. 


P11-10. Modulation of intestinal T cells following infection of macaques with live attenuated and conditionally replication-competent SIV

Crossref

Berkhout B

Klaver B

Kleibeuker W

Das AT

Page M

Robinson M

Quartey-Papafio R

Stebbings R

Manoussaka MS

Almond N

Cranage M

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P11-10. Modulation of intestinal T cells following infection of macaques with live attenuated and conditionally replication-competent SIV

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