1 página.-- Presentación de póster presentation.-- Este artículo es parte del suplemento: AIDS Vaccine 2009.Peer reviewe

Esteban, M

Heinen, P

Jacobs, B

Kibler, K

Loof, N

Melief, C

Nieto, J

Pantaleo, G

Perdiguero, B

Quakkelaar, E

Redeker, A

English

PubMed

Quakkelaar, E.

Redeker, A.

Loof, N.

Perdiguero, B.

Heinen, P.

Nieto, J.

Esteban, M.

Kibler, K.

Jacobs, B.

Pantaleo, G.

Melief, C.

Serveur académique lausannois

Enhanced expression of HIV antigens and improved antigen presentation after infection with replication competent attenuated vaccinia virus in vitro : P17-23

Perdiguero, Beatriz

Esteban, Mariano

Pantaleo, Giuseppe

Digital.CSIC

BioMed CentralPage 1 of 1(page number not for citation purposes)RetrovirologyOpen AccessPoster presentationP17-23. Enhanced expression of HIV antigens and improved antigen presentation after infection with replication competent attenuated vaccinia virus in vitroE Quakkelaar*1, A Redeker1, N Loof1, B Perdiguero2, P Heinen2, J Nieto2, M Esteban2, K Kibler3, B Jacobs3, G Pantaleo4 and C Melief1Address: 1IHB, LUMC, Leiden, Netherlands, 2CNB, CSIC, Madrid, Spain, 3Arizona State University, Tempe, AZ, USA and 4CHUV, Lausanne, Switzerland* Corresponding author    BackgroundPoxvirus vectors, particularly the attenuated and host-range restricted ALVAC, MVA and NYVAC, have beenextensively used to develop the so-called T-cell vaccines.ALVAC has been a major component of a prime/boostvaccine regimen currently tested in phase III clinical trialand MVA and NYVAC have shown good levels of immu-nogenicity in combination with DNA priming. Despitethe promising immunogenicity data, there is commonagreement that the poxvirus vector T-cell vaccines need tobe improved.MethodsIn order to improve the immunogenicity of NYVAC wehave used two strategies: a) development of attenuatedreplication competent (in human cells) NYVAC and b)deletion of poxvirus genes known to encode for proteinsthat may affect the immune response. The newly gener-ated replication competent or gene deletion NYVACmutants expressing env, gag, pol and nef have been evalu-ated for their ability to stimulate HIV-specific CD8 T-cellresponses from blood mononuclear cells of HIV-infectedsubjects. HIV-specific immune responses induced by thenew NYVAC vectors were evaluated in newly developeddirect and cross-presentation assays in vitro and comparedwith the parental replication deficient NYVAC vector.Finally, expression of gag following infection of differenttarget cells (dendritic cells and HeLa cells) with replica-tion competent and deficient NYVAC vectors was alsoevaluated.ResultsThe results obtained indicated that replication competentNYVAC vectors induce: a) improvement in direct andcross-presentation, and b) substantial increase in theexpression of gag in the infected target cells.ConclusionThis project is funded under the CAVD program.from AIDS Vaccine 2009Paris, France. 19–22 October 2009Published: 22 October 2009Retrovirology 2009, 6(Suppl 3):P305 doi:10.1186/1742-4690-6-S3-P305<supplement> <title> <p>AIDS Vaccine 2009</p> </title> <editor>Anna Laura Ross</editor> <note>Meeting abstracts – A single PDF containing all abstracts in this Supplement is available <a href="http://www.biomedcentral.com/content/files/pdf/1742-4690-6-S3-full.pdf">here</a>.</note> <url>http://www.biomedcentral.com/content/pdf/1471-2105-10-S12-info.pdf</url> </supplement>This abstract is available from: http://www.retrovirology.com/content/6/S3/P305© 2009 Quakkelaar et al; licensee BioMed Central Ltd. 

Retrovirology

P17-23. Enhanced expression of HIV antigens and improved antigen presentation after infection with replication competent attenuated vaccinia virus in vitro

Jacobs B

Kibler K

Esteban M

Nieto J

Heinen P

Perdiguero B

Loof N

Redeker A

Quakkelaar E

Pantaleo G

Melief C

Directory of Open Access Journals

P17-23. Enhanced expression of HIV antigens and improved antigen presentation after infection with replication competent attenuated vaccinia virus <it>in vitro</it>

E Quakkelaar

A Redeker

N Loof

B Perdiguero

P Heinen

J Nieto

M Esteban

K Kibler

B Jacobs

G Pantaleo

C Melief

Crossref

Springer - Publisher Connector

BioMed Central
Page 1 of 1
(page number not for citation purposes)
Retrovirology
Open AccessPoster presentation
P17-23. Enhanced expression of HIV antigens and improved antigen 
presentation after infection with replication competent attenuated 
vaccinia virus in vitro
E Quakkelaar*1, A Redeker1, N Loof1, B Perdiguero2, P Heinen2, J Nieto2, 
M Esteban2, K Kibler3, B Jacobs3, G Pantaleo4 and C Melief1
Address: 1IHB, LUMC, Leiden, Netherlands, 2CNB, CSIC, Madrid, Spain, 3Arizona State University, Tempe, AZ, USA and 4CHUV, Lausanne, 
Switzerland
* Corresponding author    
Background
Poxvirus vectors, particularly the attenuated and host-
range restricted ALVAC, MVA and NYVAC, have been
extensively used to develop the so-called T-cell vaccines.
ALVAC has been a major component of a prime/boost
vaccine regimen currently tested in phase III clinical trial
and MVA and NYVAC have shown good levels of immu-
nogenicity in combination with DNA priming. Despite
the promising immunogenicity data, there is common
agreement that the poxvirus vector T-cell vaccines need to
be improved.
Methods
In order to improve the immunogenicity of NYVAC we
have used two strategies: a) development of attenuated
replication competent (in human cells) NYVAC and b)
deletion of poxvirus genes known to encode for proteins
that may affect the immune response. The newly gener-
ated replication competent or gene deletion NYVAC
mutants expressing env, gag, pol and nef have been evalu-
ated for their ability to stimulate HIV-specific CD8 T-cell
responses from blood mononuclear cells of HIV-infected
subjects. HIV-specific immune responses induced by the
new NYVAC vectors were evaluated in newly developed
direct and cross-presentation assays in vitro and compared
with the parental replication deficient NYVAC vector.
Finally, expression of gag following infection of different
target cells (dendritic cells and HeLa cells) with replica-
tion competent and deficient NYVAC vectors was also
evaluated.
Results
The results obtained indicated that replication competent
NYVAC vectors induce: a) improvement in direct and
cross-presentation, and b) substantial increase in the
expression of gag in the infected target cells.
Conclusion
This project is funded under the CAVD program.
from AIDS Vaccine 2009
Paris, France. 19–22 October 2009
Published: 22 October 2009
Retrovirology 2009, 6(Suppl 3):P305 doi:10.1186/1742-4690-6-S3-P305
<supplement> <title> <p>AIDS Vaccine 2009</p> </title> <editor>Anna Laura Ross</editor> <note>Meeting abstracts – A single PDF containing all abstracts in this Supplement is available <a href="http://www.biomedcentral.com/content/files/pdf/1742-4690-6-S3-full.pdf">here</a>.</note> <url>http://www.biomedcentral.com/content/pdf/1471-2105-10-S12-info.pdf</url> </supplement>
This abstract is available from: http://www.retrovirology.com/content/6/S3/P305
© 2009 Quakkelaar et al; licensee BioMed Central Ltd. 


P17-23. Enhanced expression of HIV antigens and improved antigen presentation after infection with replication competent attenuated vaccinia virus 

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P17-23. Enhanced expression of HIV antigens and improved antigen presentation after infection with replication competent attenuated vaccinia virus in vitro

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