Archer, Desmond B.

Cogliati, Tiziana

Dellett, Margaret

Gu, Ping

Guduric-Fuchs, Jasenka

Ringland, Laura J.

English

PubMed

PURPOSE: The pig eye is similar to the human eye in terms of anatomy, vasculature, and photoreceptor distribution, and therefore provides an attractive animal model for research into retinal disease. The purpose of this study was to characterize retinal histology in the developing and mature pig retina using antibodies to well established retinal cell markers commonly used in rodents.METHODS: Eyes were enucleated from fetuses in the 9th week of gestation, 1 week old piglets and 6 months old adult animals. Eyeglobes were fixed and cryosectioned. A panel of antibodies to well established retinal markers was employed for immunohistochemistry. Fluorescently labeled secondary antibodies were used for signal detection, and images were acquired by confocal microscopy. Mouse retina at postnatal day (P) 5 was used as a reference for this study to compare progression of histogenesis. Most of the primary antibodies have previously been used on mouse tissue.RESULTS: Most of the studied markers were detected in midgestation pig retina, and the majority had a similar distribution in pig as in P5 mouse retina. However, rhodopsin immunolabeling was detected in pig retina at midgestation but not in P5 mouse retina. Contrary to findings in all rodents, horizontal cells were Islet1-positive and cones were calbindin-immunoreactive in pig retina, as has also been shown for the primate retina. Recoverin and rhodopsin immunolabeling revealed an increase in the length of photoreceptor segments in 6 months, compared to 1 week old animals.CONCLUSIONS: Comparison with the published data on human retina revealed similar marker distribution and histogenesis progression in the pig and human retina, supporting the pig as a valuable animal model for studies on retinal disease and repair. Furthermore, this study provides information about the dynamics of retinal histogenesis in the pig and validates a panel of antibodies that reliably detects developing and mature retinal cell phenotypes in the pig retina.</p

Ringland, Laura J

Archer, Desmond B

Queen's University Belfast Research Portal

Immunohistochemical study of pig retinal development

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Antibodies against filamentous components in discrete cell types of the mouse retina.

Axonal neurofilament-H immunolabeling in the rabbit retina.

Basic fibroblast and epidermal growth factors stimulate survival in adult porcine photoreceptor cell cultures. Invest Ophthalmol Vis Sci

Comparative analysis of progenitor cells isolated from the iris, pars plana, and ciliary body of the adult porcine eye. Stem Cells

Comparative study of the three neurofilament subunits within pig and human retinal ganglion cells.

Conditional deletion of activating protein 2alpha (AP-2alpha) in the developing retina demonstrates non-cell-autonomous roles for AP-2alpha in optic cup development. Mol Cell Biol

Development and expression of the green fluorescent protein in porcine embryos derived from nuclear transfer of transgenic granulosa-derived cells. Anim Reprod Sci

Development of the embryonic porcine neuroretina in vitro. Ophthalmic Res

Development of the outer retina in the mouse. Brain Res Dev Brain Res

Development of the retina in the porcine fetus. A light microscopic study. Anat Histol Embryol

Developmental expression of calretinin immunoreactivity in the human retina and a comparison with Molecular Vision

Developmental expression of the glial fibrillary acidic protein (GFAP) gene in the mouse retina. Cell Mol Neurobiol

Differential localization and expression of alpha and beta isoenzymes of protein kinase C in the rat retina.

Differential regulation of a glial fibrillary acidic protein-LacZ transgene in retinal astrocytes and muller cells. Invest Ophthalmol Vis Sci

Differentiation and migration of astrocyte precursor cells and astrocytes in human fetal retina: Relevance to optic nerve coloboma.

Distribution and density of mediumand short-wavelength selective cones in the domestic pig retina. Exp Eye Res

Early expression of recoverin in a unique population of neurons in the human retina. Anat Embryol (Berl)

Effects of muller cell disruption on mouse photoreceptor cell development. Exp Eye Res

Enhanced protein expressions of sortilin and p75NTR in retina of rat following elevated intraocular pressure-induced retinal ischemia. Neurosci Lett

Expression of photoreceptor-specific nuclear receptor NR2E3 in rod photoreceptors of fetal human retina. Invest Ophthalmol Vis Sci

Expression of the LIMhomeodomain protein Isl1 in the developing and mature mouse retina.

Expression patterns of calretinin, calbindin and parvalbumin and their colocalization in neurons during development of macaca monkey retina. Exp Eye Res

function, and new antibodies.

GABAA and GABAC receptors in adult porcine cones: evidence from a photoreceptor-glia co-culture model.

gene expression defines the retinal ganglion and inner nuclear layers of the developing and adult mouse retina.

Genetically engineered large animal model for studying cone photoreceptor survival and degeneration in retinitis Molecular Vision

Glial cell reactivity in a porcine model of retinal detachment. Invest Ophthalmol Vis Sci

Hippocalcin in rat retina. comparison with calbindin-D28k, calretinin and neurocalcin. Exp Eye Res

Immunocytochemical analysis of the mouse retina.

Immunocytochemical description of five bipolar cell types of the mouse retina.

Immunocytochemical localization of calretinin containing neurons in retina from rabbit, cat, and dog. Neurosci Res

In vitro analysis of a mammalian retinal progenitor that gives rise to neurons and glia. Brain Res

Isolation of progenitor cells from GFP-transgenic pigs and transplantation to the retina of allorecipients. Cloning Stem Cells

Isolation of retinal progenitor and stem cells from the porcine eye. Mol Vis

Lectin and antibody labelling of developing rat photoreceptor cells: An electron microscope immunocytochemical study.

Localization of p75 neurotrophin receptor in the retina of the adult SD rat: An immunocytochemical study at light and electron microscopic levels. Glia

Morphology of calretinin and tyrosine hydroxylase-immunoreactive neurons in the pig retina. Mol Cells

Muller cell expression of glutamate cycle related proteins and antiapoptotic proteins in early human retinal development.

Muller cells express the neuronal progenitor cell marker nestin in both differentiated and undifferentiated human foetal retina. Clin Experiment Ophthalmol

Muller glial cells express nestin coupled with glial fibrillary acidic protein in experimentally induced glaucoma in the rat retina. Neuroscience

PAX6 expression in the developing human eye.

Pax6 is required for the multipotent state of retinal progenitor cells. Cell

Photoreceptor density of the domestic pig retina. Vet Ophthalmol

Practical applications of some antibodies labelling the human retina. Histol Histopathol

Progenitor cells from the porcine neural retina express photoreceptor markers after transplantation to the subretinal space of allorecipients. Stem Cells

Recoverin, a novel calcium-binding protein from vertebrate photoreceptors. Biochim Biophys Acta

Retinal degeneration in cone photoreceptor cell-ablated transgenic mice.

Retinal pigment epithelial debridement as a model for the pathogenesis and treatment of macular degeneration.

Rod photoreceptor differentiation in fetal and infant human retina. Exp Eye Res

Selective excitotoxic degeneration of adult pig retinal ganglion cells in vitro. Invest Ophthalmol Vis Sci

Subunit dissociation and diffusion determine the subcellular localization of rod and cone transducins.

Survival and regeneration of adult human and other mammalian photoreceptors in culture.

Temporal kinetics of the light/dark translocation and compartmentation of arrestin and alpha-transducin in mouse photoreceptor cells.

The anterior lens capsule used as support material in RPE cell-transplantation. Acta Ophthalmol Scand

The brn-3 family of POU-domain factors: Primary structure, binding specificity, and expression in subsets of retinal ganglion cells and somatosensory neurons.

The intermediate filament complement of the retina: A comparison between different mammalian species.

The pig eye as a novel model of glaucoma. Exp Eye Res

Transgenic-cloned pigs systemically expressing red fluorescent protein, kusabiraorange. Cloning Stem Cells

Transplantation of full-thickness retina in the rhodopsin transgenic pig. Retina

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Immunohistochemical study of pig retinal development

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