Na publicação: Jacqueline Cavalcanti Barros, Renato Andreotti. PAG 2019. PE0246

ANDREOTTI, R.

BARROS, J. C.

CASTRO, G. M. de

GARCIA, M. V.

GIACHETTO, P. F.

NHANI JUNIOR, A.

RODRIGUES, V. da S.

English

Repository Open Access to Scientific Information from Embrapa

PE0246: Differential Gene Expression in the Cattle Tick Rhipicephalus microplus Fed on Resistant and SusceptibleCattleRhipicephalus microplus is an ectoparasite that has substantial economic impact on the cattle breeding industry, through direct effects of feeding and by transmission ofsignificant cattle diseases. The use of acaricides is the conventional form of control of the parasite, but efforts have been employed in the search for alternative forms ofcontrol. In this context, genomic tools can contribute to the understanding of molecular mechanisms to the discovery of targets that can be used in new control strategies,such as vaccine development. Insights into the components involved in ticks blood feeding, blood digestion and reproduction are essential to understanding tick-hostinteractions and development and to identify novel targets that can serve for the basis to tick control strategies.Using RNA-Seq technology, we obtained the transcriptional profiling of salivary glands, ovary and gut from R. microplus fed on resistant (Nelore), susceptible (Holstein) andcrossbreed cattle, with the aim to understand the host breed effects on tick gene expression during the tick-host interaction. A total of 484,754,177 Illumina paired-endedsequences (2x100bp) were de novo assembled using Trinity. Differentially expressed genes (DEG) between the transcriptomes of ticks fed on resistant, susceptible andcrossbreed cattle were identified by EdgeR and revealed 137, 252 and 134 DEG (FDR-adjusted p-value <0.05) in salivary glands, ovary and gut, respectively.Functional analysis using Blast2GO showed an enrichment in upregulated genes that code for proteins with transferase activity in crossbreed cattle compared to Holsteincattle at tick salivary glands. Genes that code for proteins with transferase and peptidase activity also were upregulated in crossbreed cattle fed tick gut, compared withHolstein fed ticks. Most ovarian DEG were upregulated in crossbreed cattle, including genes that code for proteins with transferase, hydrolase and with binding functions.Further analysis are planned to focus on the role of these genes to unveil the molecular bases of the tick interaction with resistant and susceptible cattle.AuthorsPoliana F GiachettoEmbrapa Informática AgropecuáriaAntonio Nhani JrEmbrapa Informática AgropecuáriaGiovanni Marques de CastroUniversidade Federal de Minas Gerais -UFMGVinícius da Silva RodriguesLaboratório de Biologia do Carrapato,Embrapa Gado de CorteMarcos Valério GarciaLaboratório de Biologia do Carrapato,Embrapa Gado de CorteJacqueline Cavalcanti BarrosEmbrapa Gado de CortePlant and Animal Genome XXVII Conference<br>(January 12- 16, 2019) https://pag.confex.com/pag/xxvii/meetingapp.cgi/Paper/357661 of 2 06/01/2020 08:22Renato Andreotti Embrapa Gado de CorteFind SimilarView Related EventsPlant and Animal Genome XXVII Conference<br>(January 12- 16, 2019) https://pag.confex.com/pag/xxvii/meetingapp.cgi/Paper/357662 of 2 06/01/2020 08:22

Differential gene expression in the cattle tick Rhipicephalus microplus fed on resistant and susceptible cattle.

http://www.alice.cnptia.embrapa.br/bitstream/doc/1118136/1/DifferentialGeneExpressionPAG.pdf

Differential gene expression in the cattle tick Rhipicephalus microplus fed on resistant and susceptible cattle.

Abstract

Similar works

Full text

Available Versions

Repository Open Access to Scientific Information from Embrapa