Twelve compounds were identified by GC.MS in the essential oil of Elyonurus muticus leaves, native in the Brazilian Pantanal. Spathulenol (18.6%), B-caryophyllene (17.9%) and camphene (11.5%) were the major components found in the oil

MARQUES, M. O. M.

POTT, A.

SAITO, M. L.

SCRAMIN, S.

English

Repository Open Access to Scientific Information from Embrapa

Essential Oil of Elyonurus muticus (Sprengel) O.Kuntze (Gramineae)Shirlei Scramin* and Maria Lúcia SaitoEmbrapa Meio Ambiente. CP 69. Jciguariihia. SP 13820-000. BrasilArnildo PottEmbrapa Pantanal. CP 109. Corumbá MS. BrasilMarcia Ortiz Mayo MarquesCentro de Genética, Biologia Molecular e Fitoquimica. CP 28. Campinas. SP. 13001-910. BrasilAbstractTwelve compounds were identified by GC/MS in the essential oil of Elyonurus muticus leaves, native in the Brazilian Pantanal. Spathulenol (18.6%), |3-caryophyl- lene (1? 9%) and camphene (11.5%) were the major components found in the oil../. Essen!. Oil Res.. 12. 298-300 (May-jun 2000)Key Word IndexElyonurus muticus, Gramineae, essential oil composition, ß-caryophyllene, camphene, spathulenol.IntroductionElyonurus muticus (Sprengel) O.Kuntze, is native in Brazilian Pantanal, Mato Grosso do Sul. It is dominant in a type of grassland, burning even green, due to the oil. This plant is not appropriate to cattle feeding, as the bitter taste is transmitted to the milk (1-3), but only the regrowth is grazed.The genus Elyonurus is common in South America, growing from Brazil to Paraguai and Argentina (4,5). E. muticus of Argentinian origin was found to contain more than 90% citral (3). The oil of E. latiflorus contained 3-0-5.0 % citral, while E. viridulus oil contained about 90% citral (4,6,7). Also, in an oil of E. rostratus (£. muticus') geranial (49%), neral (29.7%), geraniol (8.2%), and nerol (5.4%) were found (8). E. candidus, considered a variety of E. latiflorus, possessed 32.2% of neral and 60.7% of geranial (4). In one species o f Elyonurus 1,8-cineole, camphene, hydrocarbons and a sesquiterpenic alcohol were found (4). " . ■ ' C ’ vIn this paper, we are presenting the chemical composition of the oil isolated from leaves of£. muticus collected in the Pantanal of Mato Grosso do Sul.ExperimentalPlant Material and Oil Isolation: Elyonurus muticus (Sprengel) O.Kuntze was collected in April 1996 in the Brazilian Pantanal, Mato Grosso do Sul. Voucher specimen was deposited in the CPAP Herbarium, number AC Cervi 4249-•Address for correspondence1041-2905/00/0003-0298S6.00/0— ©2000 Allured Publishing Corp.Received: March 1999 Accepted: June 1999E ly o n u x v s  MCT1CUS 299Table I. Percentage composition o f the oil of E lyo nurus m u tic u s  (Sprengel) O. KuntzeCom ponents3 PercentageRI calculatedRI LitIdentificationmethodDB-1 DB-5a-thujene 0.5 912 - 931 ba-pinene 0.9 922 - 939 a,b,ccamphene 11.5 933 947 953 a,b,cmyrcene 4.5 979 990 991 a,b,climonene 0.2 1015 1027 1015 a.b.c(Z)-trimenalT 3.0 1374 1389 1395 bp-caryophyllene 17.9 1397 1418 1418 a,b,c(Z)-p-farnesene 4.5 1404 1433 1443 a,b,ca-humulene 4.0 1428 1452 1454 a,b,cspathulenol 18.6 1542 1576 1576 bírans-sesquisabinene hydrate 4.8 1545 1580 1580 bcedren-8-en-9a-ol 2.8 1598 1641 1644 bnot identified 26.8Identification Methods: a -  GC/MS library search; b = MS. Lit (9-11); c = co-injection with authentic sample; a Components are listed according to their elution on DB-1 and DB-5 capillary column; r also known as (Zl-2.5.9-trirnethvl-4.9-decadienal____________________________________________________Essential oil (1.8 mL) was obtained from 408 g o f fresh leaves by hydrodistillation in a Clevenger- type apparatus, for 8 h.Analysis: The oil was analyzed by GC/MS (Shimadzu, model QP-5000) equipped with an electron impact (70 eV) detector, and a (25 mm x 0.25 mm x 0.25 Hm) fused silica capillary column (DB-1). The carrier gas was helium (1.7 mL/min); 1 (iLo f the sample was injected. The programmed temperature was 50°-l60°C, 2°C/'min; 160°C (min); l60°-280°C, 10°C/min. Injector temperature was 240°C and detector, 230°C, split 1/35. The retention index was obtained with a DB-1 column, in the same conditions of the analyze, and in the DB-5 (30 mm x 0.25 mm x 0.25 Hm) column, with programmed temperature o f 60°-240°C, 3°C/min; split 1/20, injector and detector at 220°-240°C, respectively, maintaining the helium flow, constant (1 L/min), by programmed pressure. The oil components were identified by comparison of their retention index, MS literature data, equipment database and co-injection of authentic samples.Results and DiscussionThe compounds identified in the oil are listed in Table I. Among the 18 compounds found, sesquiterpenes represented 52.6% and monoterpenes 17.7%. Spathulenol (18.6 %) and P-caryophyllene (17.9%) were the major components; the monoterpenes camphene (11.5%) and p-myrcene (4.5%) were also present.In other studies, species of Elyonurus were found to contain high amount of aldehydes as citral, citronellal, neral and geranial {citral). In this Brazilian species these compounds were not found. The material was at mature stage. There might be seasonal variation in oil content.References1. E. B. Pott, P.A.R. Brum, I. L. Almeida, J. A. Comastri F. O. and A. Pott, Nutrição mineral de bovinos de corte no Pantanal Mato- Grossense. Levantamento de macronutrientes na sub-região de Aquidauana. Pesquisa Agropecuária Brasileira, 24, 1381-1395 (1989).2. J. A Comastri F. O. and A. Pott, Introdução e avaliação de forrageiras em "caronal” na sub-região de Nhecolândia, Pantanal Mato-Grossense. Pesquisa Agropecuária Brasileira, 29, 1637-1650, (1994).3. A. Burkart, Flora ilustrada de Entre Rios (Argentina). Parte II. Gramineas: la família botânica de los Pastos. Buenos Aires, INTA, pp 455-456, (Colección Científica del INTA, t.6, 2), (1969).300 S c r a m  in  e t  a l .4. Ci. A. Fester. J. Retamar, A. Ricciardi and L. Fonseca Romero. Algitnasesencias volatiles. ('Esencias del Sordeste Argentino). Rev. Fac. Ing. Quim. Cnir. .\'ac. Lit. Santa Fe, 28, 9-17 (1959).5. Ci. A Fester, Observaciones adicionales sobre esencias volatiles argentinas. Bol. Acad. Nac. Ci. de Cordoba (Argentina), 41. 197-210 (I960).6. A. L. Montes, Produccion deplantasaromaticas natwas. IDIA: Informativo de Investigaciones Agropeacuarias, Buenos Aires. 211. 103-111 (1965).7. V.F.W. Hefendeh! and L. R. Fonseca. Analysis o f the essential oil o f Elyonums viridulus. Planta Med.. 30. 135-140 (1976).8. K. Dellacassa. P. Menendez and E. Soler, Composition o f  the essential oil o f  Elyonums rostratus (E. nmticits). Fssenze Deriv. Agrum., 58. 207-210 (1988): Chem. Abstr.. 110. 218. 772 (1989).9. R. P. Adams. Identification o f Essential Oil Components by Gas Chmmatograph/.Mass Spectmscopy. Allured Publ. Corp.. Carol Stream. IL (1995).10. A. A. Swigar and R. M. Silverstein. Monoteipenes -  Infrared. Mass. 'H  NMR. and 11C XMR Spectra and Kovat Indices. Aldrich Chem. Co.. Milwaukee. WI (1981J.11. F. W. McLafferty and D. B. Staufer. The WHey/NBS Registry o f  Mass Spectral Data. Vol. 1-6. Wiley, New York (1989).

Essential oil of Elyonurus muticus (Sprengel) O. Kuntze (Gramineae).

Twelve compounds were identified by GC.MS in the essential oil of Elyonurus muticus leaves, native in the Brazilian Pantanal. Spathulenol (18.6%), B-caryophyllene (17.9%) and camphene (11.5%) were the major components found in the oil.Made available in DSpace on 2020-01-20T18:10:17Z (GMT). No. of bitstreams: 1
ScraminEssential.pdf: 103375 bytes, checksum: 2c2f556bfd6d48d962fd32abae36e0b0 (MD5)
  Previous issue date: 200

LAReferencia - Red Federada de Repositorios Institucionales de Publicaciones Científicas Latinoamericanas

http://www.alice.cnptia.embrapa.br/bitstream/doc/1119036/1/ScraminEssential.pdf

Essential oil of Elyonurus muticus (Sprengel) O. Kuntze (Gramineae).

Abstract

Similar works

Full text

Available Versions

Repository Open Access to Scientific Information from Embrapa

LAReferencia - Red Federada de Repositorios Institucionales de Publicaciones Científicas Latinoamericanas