ARRUDA, P.

CANESIN, L. E. C.

DANTE, R. A.

GERHARDT, I. R.

OLIVEIRA, R. S.

RIAÑO PACHÓN, D. M.

English

Repository Open Access to Scientific Information from Embrapa

PE1164: Sequencing of Vellozia Spp. Genomes to Understand Drought Tolerance and Phosphorus Acquisition inAngiospermsVelloziaceae are an angiosperm family that contains the most desiccation-tolerant species (approximately 200 out of 270 species). These species are distributed among fivegenera: Acanthochlamys, Xerophyta, Barbacenia, Barbaceniopsis and Vellozia. More than 80% of the Velloziaceae species occur in South America, where the greatestmorphological diversity is also found. The genus Vellozia comprises both desiccation-tolerant and non-desiccation-tolerant species, offering an excellent model for studyingthe evolution of desiccation- and drought-tolerance traits on plant genomes. Desiccation-tolerant Vellozia nivea and evergreen Vellozia intermedia are both drought-tolerant,endemic to the Brazilian campos rupestres (rupestrian grasslands) and highly adapted to their extreme conditions. These are characterized by an extended dry season fromMay to September, high solar radiation and rocky, shallow, nutrient-poor soils with particularly very low phosphorus (P) availability. Unlike most model plants that come fromenvironments where nitrogen (N) is the major limiting nutrient, Vellozia spp. have evolved in an environment where P is the key limiting nutrient, becoming a valuable modelfor crops cultivated in tropical soils.Here we report the genome assemblies of the two Vellozia using a PacBio based, single-molecule real-time (SMRT) sequencing approach. We generated over 50 Gb of longreads for each species, representing ~100x coverage. We have also generated Illumina short reads at a depth of 60x. The genome size was determined by flow cytometry,resulting in 510 Mb for V. intermedia and 477 Mb for V. nivea. PacBio reads were assembled using Canu, and Illumina short reads were only used to polish the assemblieswith Pilon. Construction of primary haploid assemblies were performed using HaploMerger2. The V. intermedia assembly spanned 452.7Mb across 1541 contigs with a contigN50 of 756.4 kb. The V. nivea assembly is smaller, at 323.9 Mb with 1521 contigs and an N50 of 671.7 kb.Vellozia species are self-incompatible, and their genomes are highly polymorphic. The availability of high-quality genomes are crucial to allow the comparative analysis ofspecific genes and genes families encoding proteins characteristically involved with several molecular processes underlying responses to stresses. In order to achieve high-quality genomes, Chicago and Hi-C libraries preparation and sequencing are underway.AuthorsIsabel R. GerhardtEmbrapa/GCCRCRicardo A. DanteEmbrapa/GCCRCDiego M. Riaño PachónCENA, USPLucas Eduardo Costa CanesinUnicamp/GCCRCRafael S. OliveiraUniversity of Campinas - UNICAMPPaulo ArrudaUnicamp/GCCRCPlant and Animal Genome XXVII Conference<br>(January 12- 16, 2019) https://pag.confex.com/pag/xxvii/meetingapp.cgi/Paper/372521 of 2 06/01/2020 09:16Find SimilarView Related EventsPlant and Animal Genome XXVII Conference<br>(January 12- 16, 2019) https://pag.confex.com/pag/xxvii/meetingapp.cgi/Paper/372522 of 2 06/01/2020 09:16

Sequencing of Vellozia Spp. genomes to understand drought tolerance and phosphorus acquisition in angiosperms.

http://www.alice.cnptia.embrapa.br/bitstream/doc/1118167/1/SequencingVelloziaPAG.pdf

Sequencing of Vellozia Spp. genomes to understand drought tolerance and phosphorus acquisition in angiosperms.

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