ANDRADE, T. N.

CATARINO, R. P. F.

GOMES, R. J.

HATA, N. N. Y.

MANDARINO, J. M. G.

ROSA, M. F.

SPINOSA, W. A.

English

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DETERMINING THE BEST CONDITIONS FOR SIMULTANEOUS" PRODUCTION OF ACETIC ACID AND BACTERIAL CELLULOSE BY#949?" Komagataeibacter sp. V-05 USING SOYBEAN MOLASSEScomes, R. .|-; 1-urrn, ll- u- v.; cnrnnmo, n. P. |=.; mosses, ‘I’. n-; umoanmo, .1- u. c_; ROSA, u- |=.; SPINOSIL w. A.$0TelniilicoINOVAQAO E BIOTECNOLOGIAPalavras chiveAcetic Acid Bacteria, fermentation,CO—PRODUCT, BOX BEHNKENPara mais infonnacfia, acesse o sin dowillThe bacterial genus Komagataeibacter comprises mesophilic microorganisms, Gram negative, catalase positive, strictly aerobic and inrod-shaped form. They belong to the family Acetobacieraceae presenting high capacity in oxidizing ethanol to acetic acid, beingtherefore used in the production of vinegar. These bacteria are also used in the production of bacterial cellulose, a polymer withbiomedical applications. The soybean molasses is an industrial co-product, generated in the production of soy protein concentratefrom defatted soybean meal. it is a brown syrup that contains a high percentage of carbohydrates (about 70% soluble solids), as wellas lipids, proteins, fibers and minerals. The objective of this work was to detemiine the best conditions for the simultaneousproduction of acetic acid and bacterial cellulose by an acetic acid bacteria previously isolated from a vinegar industry and identified asKomagataeibacter sp. W05, using as a fennentation substrate the coproduct soybean molasses. Box Behnken factorial design withthree factors and three levels (X1 — °Brix: 15, 20 and 25; X2 - ethanol concentration (96): 1, 3,5 and 6; X3 - fennentation time (days): 6 ,1 0 and 14), was used, totaling 15 randomly distributed experiments. At the end of the femientation time, the acidity was measured bytitration with 0.1 M Na0H and expressed as ‘J5 acetic acid. The membranes formed on the surface of each medium were heated at 80 “C I 30 min. with alkaline solution (1 M Na0H) and then, rinsed to neutral pH with distillated water. The membranes were dried at 105 ' Cfor detemiination of the respective dry weight (g/L). After the experiments, it was observed that the production of acetic acid wasfavored in higher concentrations of ethanol (6%) and lower °Brix [1 5), occurring increase until the 12th day of fermentation. Underthese conditions, the maximum acidity yield (3.65%) was obtained. On the other hand, bacterial cellulose production was favored inlower concentrations of ethanol (1%), longer femientation time (14 days), being indifferent in relation to Ute "Brix of the soybeanmolasses. A maximum yield of 11.4 g L-1 was achieved. It was concluded that soybean molasses can be reused as a culture mediumfor the production of acetic acid and bacterial cellulose. However, the fermentation parameters such as °Brix, ethanol concentrationand fennentation time should be taken into consideration separately for a better yield of each microbial metabolite.6flfi{SottwiIe|:iara Ciéncia

Determining the best conditions for simultaneous production of acetic acidand bacterial celulose by Komagataeibacter sp. V-05 using soybean molasses.

http://www.alice.cnptia.embrapa.br/bitstream/doc/1099040/1/Determining.pdf

Determining the best conditions for simultaneous production of acetic acidand bacterial celulose by Komagataeibacter sp. V-05 using soybean molasses.

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