CORREA, D. S.

DAIKUZONO, C. M.

GOBBI, A. L.

MANZOLI, A.

OLIVEIRA JR, O. N.

PIAZZETTA, M. H.

RIUL JR, A.

SHIMIZU, F. M.

Portuguese

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Microfluidic electronic tongue to detect gliadinin foodstuffCristiane Margarete Daikuzono1, Flávio Makoto Shimizu1, Antonio Riul Jr.2,Alexandra Manzoli3, Maria Helena Piazzetta4, Angelo Luiz Gobbi4, Daniel SouzaCorrêa3, Osvaldo Novais Oliveira Jr11Instituto de Física de São Carlos/Universidade de São Paulo, 2Instituto de FísicaGleb Wataghin, 3Embrapa Instrumentação Agropecuária - São Carlos, 4CentroNacional de Pesquisa em Energia e Materiaise-mail: daikuzonocm@gmail.comThe increasing number of reported cases of celiac disease motivates research forthe food industry to obtain gluten-free food and detect gluten. Devices for suchdetection must  be portable  and easy  to  use  as  they will  be  handled by  theconsumers themselves. A suitable option is to employ the microfluidic electronictongue concept[1].  In this study, we fabricated e-tongues to detect gliadin, aprotein  of  gluten  present  in  foodstuffs  and  responsible  for  the  disease.  Thesensing  units  were  produced  by  adsorbing  nanostructured  films  fromsemiconducting  materials  using  the  dynamic  self-assembly  technique(layer-by-layer)[2]onto microchannels containing interdigitated microelectrodes.Impedance spectroscopy was employed to detect gliadin in synthetic samples,where gliadin was added to an ethanol 70%, and in real food samples. Informationvisualization methods were used to process the data, which helped distinguishbetween samples containing different concentrations of gliadin, in addition todetermining whether gliadin was present in foodstuff samples. Significantly, nofalse positives or negatives were observed. The change in the impedance spectramay  be  associated  with  gliadin  adsorption  onto  the  nanostructured  films,according  to  data  from  Polarization-modulated  infrared  reflection  absorptionspectroscopy (PM-IRRAS) and UV-Vis spectroscopy.References[1]C.M.Daikuzono,et al.,“Microfluidic electronic tongue,” Sens. Actuators B Chem.,207,1129–1135,2015.[2]H.-J.Kim,K.Lee,S.Kumar,  and  J.  Kim,“Dynamic  sequential  layer-by-layerdeposition method for fast and region-selective multilayer thin film fabrication,”Langmuir,21,8532–8538,2005.AcknowledgementCAPES,FAPESP,CNPq,INEO.1304XIV Brazil MRS Meeting - Rio de Janeiro, from 27 September to 01 October, 2015 1305XIV Brazil MRS Meeting - Rio de Janeiro, from 27 September to 01 October, 2015

Microfluidic electronic tongue to detect gliadin in foodstuff.

http://www.alice.cnptia.embrapa.br/bitstream/doc/1036625/1/Microfluidicelectronictonguetodetectgliadininfoodstuff..pdf

Microfluidic electronic tongue to detect gliadin in foodstuff.

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