The aim of this study we evaluated the attractiveness and the leaf injury caused by A. gemmatalis larvae in bean genotypes

AIALA, J. G. E. A. R.

BERNARDES, G. A. P.

MELVILLE, C. C.

NOGUEIRA, L.

OLIVEIRA, N. T.

SOUZA, T. L. P. O.

English

Repository Open Access to Scientific Information from Embrapa

ATTRACTIVENESS AND INJURY OF Phaseolus vulgaris L. GENOTYPES BY Anticarsia gemmatalis HÜBNER (LEPIDOPTERA: EREBIDAE)  J. G. E. A. R. Aiala1, L. Nogueira1*, G. A. P. Bernardes1, C. C. Melville2, N. T. Oliveira3, T. L. P. O. Souza4  1 Univ. Estadual de Goiás, UEG, Laboratório de Entomologia Agrícola, Rod. GO 330, Km 241, s/n°, Ipameri, GO, 75780-000, Brazil; 2Univ. Est. Paulista, UNESP/FCAV, Via de Acesso Prof. Paulo Donato Castellane s/nº, Jaboticabal, SP, 14884-900, Brazil; 3Univ. Federal de Lavras, Lavras, MG, 37200-000, Brazil; 4Embrapa Arroz e Feijão, Santo Antônio de Goiás, GO, 75375-000, Brazil; *corresponding author: lucianonogueiraagro@gmail.com  INTRODUCTION The Anticarsia gemmatalis Hübner (Lepidoptera: Erebidae) is the most defoliator pest, causing economic damage to many species of crop plants, including preferentially, soybeans – [Glycine max (L.) Merrill] and common bean - Phaseoulus vulgaris L. (Herzog; Todd, 1980; Panizzi et al., 2004). These crops are extensively cultivated in Brazil, thus forming very simplified and vulnerable agroecosystems, resulting in problems such as the excessive and indiscriminate use of pesticides, with adverse ecological consequences to the environment. In an attempt to diminish pesticide use in cropping systems, alternative control methods have been investigated, and host plant resistance is one of them (Boiça Júnior et al., 2014). Searching genotypes that express tolerance and/or resistance to insect pest represents an important step for plant breeding.   Thus, the aim of this study we evaluated the attractiveness and the leaf injury caused by A. gemmatalis larvae in bean genotypes.   MATERIAL AND METHODS Assays were conducted in the agricultural entomology Laboratory, UEG, Ipameri, GO, Brazil, under environmentally controlled conditions. The following common bean genotypes were evaluated for resistance to A. gemmatalis larvae: BRS Estilo, BRS Requinte, BRS Ametista, BRSMG Realce e BRS FC402.   The experiment consisted of free-choice and no-choice assays setup in completely randomized design with 10 replications each. For the free-choice test, leaf disks (2.2 cm diameter) prepared from the respective genotypes were arranged in glass arenas (14.0 cm diameter) lined with moistened filter paper, where two neonate larvae A. gemmatalis was released per genotype, totaling 10 larvae per arena. In the no-choice test, one leaf disk was placed in each Petri dish (9.0 cm diameter) two neonate larvae. In both tests, leaf disk attractiveness was recorded 72 h after the larvae were released. To evaluate leaf injury caused by the larvae, percentage of estimated injury ranging from 0 to 100% was assigned for uninjured leaf discs and fully injured leaf discs, respectively.   Data recorded from leaf disc attractiveness percentage and leaf injury percentage were transformed into arcsine (x/100)1/2. Next, data were subjected to analyzed for normality (Levene’s test) and variance homogeneity (Shapiro-Wilk test). Data were subjected to analysis of variance (ANOVA), and means were compared by Tukey’s test (P < 0.05).   RESULTS AND DISCUSSION The bean genotypes evaluated interfered in attractiveness and injuries caused by A. gemmatalis larvae. In the free-choice test, the percentage of A. gemmatalis larvae attracted was significantly lower for BRSMG Realce genotypewhen compared to the others; no difference was found among 183other tested genotypes (Fig. 1A). The lowest percentages of injury were verified  in the BRSMG Realce and BRS Estilo. The highest injuries were observed in the BRS Requinte genotype (Fig. 1B).                         Figure 1 Attractiviness and injury percentage of common bean genotypes to larvae Anticarsia gemmatalis in free-choice (A, B) and no-choice test (C, D). Bars with diferents letters are significantly different by Tukey test at ** 1% and *5% probability.   In the no-choice test, the percentage of larvae attracted was highest in the BRS Estilo genotype (Fig. 1C). The BRSMG Realce was the least injured of A. gemmatalis (Fig. 1D).   The results illustrate that there was a direct relationship between the lower attractiveness of BRSMG Realce to the larvae and the lower injury percentage recorded in this genotype (Fig. 1). This leads us to infer that the genotype BRS Realce has compounds that may have inhibited attractiveness and consequently the feeding of the larvae. In addition, the lower preference for the BRSMG Realce may be linked to physical causes (leaf color).  The leaves of this genotype have a light green color, while the others have dark green pattern. Additionally, we cannot rule out morphological factors (presence of trichomes) and the possible effect of chemical substances (volatile) that may have acted as deterrents to larvae feeding preference.   This study shows that BRSMG Realce genotype presents characteristics resistance in the non-preference category for feeding to A. gemmatalis.  REFERENCES  BOIÇA JÚNIOR, A. L., et al. Feeding preference and development of Cerotoma arcuata (Olivier) (Coleoptera: Chrysomelidae) on soybean. Pesquisa Agropecuária Tropical, 44: 238-245. 2014. HERZOG, D. C.; TODD, J. H. Sampling velvetbean carterpillar on soybean. In: KOGAN, M. et al. Sampling methods in soybean entomology. New York: Springer-Verlag, 1980. p. 107-140. PANIZZI, A. R. et al. Survivorship, larval development and pupal weight of Anticarsia gemmatalis (Hübner) (Lepidoptera: Noctuidae) feeding on potential leguminous host plants. Neotropical Entomology 33: 563-567. 2004.  184

Attractiveness and injury of Phaseolus vulgaris L. genotypes by Anticarsia gemmatalis Hübner (Lepidoptera: Erebidae).

http://www.alice.cnptia.embrapa.br/bitstream/doc/1092201/1/CNPAF2018bicp183.pdf

Attractiveness and injury of Phaseolus vulgaris L. genotypes by Anticarsia gemmatalis Hübner (Lepidoptera: Erebidae).

Abstract

Similar works

Full text

Available Versions

Repository Open Access to Scientific Information from Embrapa