ALMEIDA, Z. G.

BASTOS, C. S.

CAIXETA, C. F.

MONNERAT, R. G. M.

PRACA, L. B.

QUEIROZ, E. S. M.

QUEIROZ, P. R. M.

SABIÁ JÚNIOR, E. F.

English

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XI SIRGEAC Simposio Internacional de Recursos Genéticos para las Américas y El Caribe268SELECTION STRAINS OF Bacillus thuringiensis TOXIC TO Helicoverpa armigera AND POTENTIAL FOR PLANT GROWTH PROMOTIONCAIXETA, C. F.1; ALMEIDA, Z. G.2; SABIÁ JÚNIOR, E. F.2; PRAÇA, L. B.2; QUEIROZ, P. R. M3; QUEIROZ, E. S. M.3; BASTOS, C. S.4; MONNERAT, R. G.21Universidade  de  Brasília/FAPDF/Embrapa  Recursos  Genéticos  e  Biotecnologia   carlaferreiracaixeta@gmail.com;  2EmbrapaRecursos Genéticos e Biotecnologia – rose.monnerat@embrapa.br; 3Convênio IMAmt/Embrapa Recursos Genéticos e Biotecnologiaericamartins@imamt.com.br ; 4  Universidade de Brasília – cschetino@unb.brAbstratHelicoverpa armigera (Hübner, 1808) (Lepidoptera: Noctuidae) is an extremely polyphagous species, feeding from different types of crops with economic importance and has great adaptability to various  climatic conditions (Srivastava et al., 2010; Bueno et al., 2014). Bacillus thuringiensis is a cosmopolitan bacteria that express different proteins with entomopathogenic activity, and may act as an important biological control agent, besides being capable to colonize the interior of plants, promoting its growth (Bravo et al., 1998; Praça et al., 2012). To know more and explore the potentials of this microorganism, the aimed of this work to select 100 strain of B. thuringiensis wich toxicity to H. armigera and potential for plant growth promotion. The strains were evaluated in vitro through a selective bioassay, the capacity of secondary metabolites to promote plant growth and the presence of genes. Twenty three strains showed 100% the mortality against H. armigera. Some strains exhibited the different profile proteins and amplicons for the cry1 and cry2 gene (table1). In vitro detection, showed that no strain had the siderophore production capability, phosphate solubiliation and biological fixation of nitrogen, only in the production of índole acetic acid (table2). 45 of the 100 analyzed strains showed the expected amplicons for all the studied genes for the acid phosphatase, siderophore and genes involved in the biosynthesis of IAA hormone of ipdC, iam1 and iam2. B. thuringiensis can be used in biological control against H. armigera and has a great potencial to promote plant growth, creating new perspectives for agronomic use.Keywords: Biological control, secondary metabolite, genetic diversity.

Selection strains of Bacillus thuringiensis toxic to Helicoverpa armigera and potential for plant growth promotion.

http://www.alice.cnptia.embrapa.br/bitstream/doc/1084985/1/resumenessirgeac268268.pdf

Selection strains of Bacillus thuringiensis toxic to Helicoverpa armigera and potential for plant growth promotion.

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