GONCALVES, S. B.

MACHADO, F.

PACHECO, T. F.

STEFANINI, R.

English

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05/12/2017 39th Symposium on Biotechnology for Fuels and Chemicalshttps://sim.confex.com/sim/39th/meetingapp.cgi/Paper/33773 1/1M44: A factorial design analysis of growth kinetics of Pichia stipitisPAPERMonday, May 01, 20176:00 PM - 8:00 PMSan Francisco Marriott Marquis - Yerba Buena Salons 9, B2 Lower LevelPichia stipitis is one of the most important host organisms used for high-level heterologous protein expression due to its capacity to promoteproper protein folding and post-translational modification, especially for proteins from eukaryotic organisms. For this reason, asadvancement of research in lignocellulosic ethanol occurs, Pichia stipitis has increasingly being used for expression of enzymes used incellulose hydrolysis. In order to understand some of the factors that may affect the growth kinetics of Pichia, a 2 factorial design experiment,with three replicates in the central point, was performed to study the effects of inoculum concentration, agitation and pH of medium on thegrowth kinetics of Pichia stipitis. The effect of those three factors was studied on the yields of biomass and ethanol productions, as well asglucose consumption. Results showed that after 24 h of fermentation, the initial concentration of inoculum was significant in determining thebiomass and ethanol yield. According to results, an increase in the inoculum meant an increase in biomass yield, and a decrease in ethanolyield. Concerning the glucose consumption rate, all of the factors, along with its binary and tertiary interactions, showed to be significant atsome point during fermentation, with inoculum and agitation being the most significant. Inoculum concentration was the only factor thataffected all of the kinetics parameters studied, and it was the most significant of all the factors.3 AuthorsRenan StefaniniThályta Fraga PachecoSilvia B. GonçalvesFabricio MachadoUniversidade de BrasíliaEmbrapaEmbrapaUniversidade de BrasíliaView Related EventsDay: Monday, May 01, 2017

M44: a factorial design analysis of growth kinetics of Pichia stipitis.

http://www.alice.cnptia.embrapa.br/bitstream/doc/1081678/1/ComprovacaoAINFOSilvia39thSymposiumonBiotechnologyforFuelsandChemicals.pdf

M44: a factorial design analysis of growth kinetics of Pichia stipitis.

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