Multiple-locus variable-number tandem repeats analysis improves surveillance and outbreak investigations

Lindstedt, Bjørn-Arne

Nielsen, Eva Møller

Sørensen, Gitte

Torpdahl, Mia

Emerging Infectious Diseases

English

PubMed

In Denmark, as part of the national laboratory-based surveillance system of human enteric infections, all Salmonella Typhimurium isolates are currently subtyped by using phage typing, antimicrobial resistance profiles, and pulsed-field gel electrophoresis (PFGE). We evaluated the value of real-time typing that uses multiple-locus-number tandem-repeats analysis (MLVA) of Salmonella enterica serotype Typhimurium (S. Typhimurium) to detect possible outbreaks. Because only a few subtypes identified by PFGE and phage typing account for most infections, we included MLVA typing in the routine surveillance in a 2-year period beginning December 2003. The 1,019 typed isolates were separated into 148 PFGE types and 373 MLVA types. Several possible outbreaks were detected and confirmed. MLVA was particularly valuable for discriminating within the most common phage types. MLVA was superior to PFGE for both surveillance and outbreak investigations of S. Typhimurium

Mia Torpdahl

Gitte Sørensen

Bjørn-Arne Lindstedt

Eva Møller Nielsen

Directory of Open Access Journals

Tandem Repeat Analysis for Surveillance of Human Salmonella Typhimurium Infections

In Denmark, as part of the national laboratory-based surveillance system of human enteric infections, all Salmonella enterica serotype Typhimurium isolates are currently subtyped by using phage typing, antimicrobial resistance profiles, and pulsed-field gel electrophoresis (PFGE). We evaluated the value of real-time typing that uses multiple-locus variable-number tandem-repeats analysis (MLVA) of S. Typhimurium to detect possible outbreaks. Because only a few subtypes identified by PFGE and phage typing account for most infections, we included MLVA typing in the routine surveillance in a 2-year period beginning December 2003. The 1,019 typed isolates were separated into 148 PFGE types and 373 MLVA types. Several possible outbreaks were detected and confirmed. MLVA was particularly valuable for discriminating within the most common phage types. MLVA was superior to PFGE for both surveillance and outbreak investigations of S. Typhimurium

Online Research Database In Technology

Alfalfa seed decontamination in a Salmonella outbreak. Emerg Infect Dis.

Amultistate outbreak of Salmonella enterica serotype Newport infection linked to mango consumption: impact of waterdip disinfestation technology. Clin Infect Dis.

Annual report on zoonoses in Denmark 2004. Copenhagen: Ministry of Food, Agriculture and Fisheries;

Antigenic formulas of the Salmonella serovars. Paris: Institut Pasteur;

Bacteriophage-typing designations of Salmonella Typhimurium.

Consensus guidelines for appropriate use and evaluation of microbial epidemiologic typing systems. Clin Microbiol Infect.

Cost-effective application of pulsed-field gel electrophoresis to typing of Salmonella enterica serovar Typhimurium. Appl Environ Microbiol.

Distribution of molecular subtypes within Salmonella enterica serotype Enteritidis phage type 4 and S. Typhimurium definitive phage type 104 in nine European countries, 2002–2004: results of an international multi-centre study. Epidemiol Infect.

DNA fingerprinting of Salmonella enterica subsp. enterica serovar Typhimurium with emphasis on phage type DT104 based on variable number of tandem repeat loci.

DNAfingerprinting of Shiga-toxin producing Escherichia coli O157 based on multiple-locus variable-number tandem-repeats analysis (MLVA). Ann Clin Microbiol Antimicrob.

Establishment of a universal size standard strain for use with the PulseNet standardized pulsed-field gel electrophoresis protocols: converting the national databases to the new size standard.

Harmonization of the multiple-locus variable-number tandem repeat analysis method between Denmark and Norway for typing Salmonella Typhimurium isolates and closer examination of the VNTR loci.

Molecular characterisation of an outbreak strain of multiresistant Salmonella enterica serovar Typhimurium DT104 in the UK. Clin Microbiol Infect.

Molecular epidemiological techniques for Salmonella strain discrimination. Front Biosci.

Molecular typing of Salmonella enterica serovar Typhi isolates from various countries in Asia by a multiplex PCR assay on variable-number tandem repeats.

Multilocus variable-number tandem repeat analysis distinguishes outbreak and sporadic Escherichia coli O157:H7 isolates.

Multiple-locus variable-number tandem-repeats analysis of Salmonella enterica subsp. enterica serovar Typhimurium using PCR multiplexing and multicolor capillary electrophoresis.

Porsbo LJ. A regional outbreak of S. Typhimurium and identification of the source using MLVA typing. Euro Surveill.

PulseNet: the molecular subtyping network for foodborne bacterial disease surveillance, United States. Emerg Infect Dis.

Salmonella surveillance: a global survey of public health serotyping. Epidemiol Infect.

Salmonella Typhimurium DT104 outbreak linked to imported minced beef, Norway, October–November

Second generation subtyping: a proposed PulseNet protocol for multiple-locus variable-number tandem repeat analysis of Shiga toxin–producing Escherichia coli O157 (STEC O157). Foodborne Pathog Dis.

The role of short sequence repeats in epidemiologic typing. Curr Opin Microbiol.

The Salm-gene project—a European collaboration for DNA fingerprinting for. Euro Surveill.

Variable number of tandem repeats in Salmonella enterica subsp. enterica for typing purposes.

http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2725892

Tandem Repeat Analysis for Surveillance of Human Salmonella Typhimurium Infections

Abstract

Similar works

Full text

Available Versions

Directory of Open Access Journals

Online Research Database In Technology