FREITAS ASTUA, J.

LE, P.

LIN, Y. C.

MENDONÇA, R. S. de

NAVIA, D.

NOVELLI, V. M.

NUNES, M. A.

SINICO, T. E.

VAN DE PEER, Y.

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8th SYMPOSIUM OF THE EUROPEAN ASSOCIATION OF ACAROLOGISTSJuly,11th -15th 201651Genetics and Evolutionary AcarologyBeating DNA out of mitesAmal Mohsan H. AL-KHALIFY1, Michael HEETHOFF2, Henk R. BRAIG11) School of Biological Sciences, Bangor University, Bangor LL57 2UW, Wales, UK  2) Ecological Networks, Tech-nical University of Darmstadt, Schnittspahnstraße 3, 64 287 Darmstadt, Germany. Email: bsp40d@bangor.ac.ukDetails of the methods for the DNA extraction of mites have become more important with an increased interest in whole genome sequencing using next generation sequencing techniques on one side and the advancement of molecular techniques in forensic acarology on the other side.  Currently one of the most used methods for homogenisation of microarthropods is bead beating employing small grinding or cutting beads in a shaking micro-tube homogenizer (BeadBug Benchmark Scientific).  A wide variety of bead materials were investigated (glass, garnet, zirconia, steel, and carbide).  The influence of different bead sizes (0.1 – 3.0 mm), different densities of the bead material (2.5 – 14.9 g/cm3), and different shapes of the beads (round balls or sharp-edged particles) on the quantity and quality of DNA extracted from mites was systematically investigated using the Qiagen DNeasy Blood & Tissue kit. The comparison was performed with four mite species: Tyrolichus casei (Acaridae) as a future model lab or food mite, Tyrophagus putrescentiae (Acaridae) as a model stored-product mite, Dermatophagoides farinae (Pyroglyphidae) as a model house dust mite, and Archegozetes longisetosus (Trhypochthoniidae) as a model soil mite.  Genome annotation of the flat mite Brevipalpus yothersi Baker (Tenuipalpidae)Valdenice M. NOVELLI1, M. Andréia NUNES1, Renata S. de MENDONÇA2, Juliana FREITAS-ASTÚA3, Thais Elise SINICO1, Yao-Cheng LIN4, Phuong LE4, Yves VAN DE PEER4, Denise NAVIA21) Centro APTA Citros Sylvio Moreira-IAC, Cordeirópolis, SP, Brazil 2) Embrapa Recursos Genéticos e Biotecno-logia, Parque Estação Biológica, final W5 Norte, Brasilia, DF, Brazil 3) CNPMF-Embrapa, Cruz das Almas, BA, Brazil 4) Ghent University, Belgium. Email: denise.navia@embrapa.br The flat mite Brevipalpus yothersi Baker, previously erroneously named as Brevipalpus phoenicis (Geijskes), was recently resurrected and redescribed into the B. phoenicis sensu stricto group. This species is one of the most important pests among the flat mites (Tenuipalpidae); these mites are highly polyphagous and their major importance is due to the transmission of numerous plant viruses- the Brevipalpus transmitted virus, BTV`s- which can seriously affect crops such as citrus, coffee, passion fruit and ornamental plants. Among them, the Citrus leprosis virus (CiLV) has been considered a key virus for the citrus production in some countries of South and Central America, as well as of the Caribbean. Due of their importance as vectors several studies have been con-ducted in order to understand the virus-vector interactions, also considering the diversity of species that may be involved. Advances in resources for genomic analyses are essential to the functional biology knowledge, however genomic data about plant mites remains limited. Here we present some features from the initial genomic analyses of assembly of the B. yothersi genome. The assembly data was performed from 454 reads and Illumina reads (paired-end and mate-paired). To successful annotation and determine the assembly quality different tools were used- Newbler, CLC genomics, and SSPACE. These analysis resulted in 849 scaffolds and the cumulative size is 72.286 Mb. Comparisons using the spider mite Tetranychus urticae Koch genome data showed that 6.4% of the total size of B. yothersi scaffolds is homologous to the known repeats from the spider mite library. Although the coding sequence of the flat mite can be considered significantly different from that of T. urticae (<80% nucle-otide identify), the predicted proteins show good alignment coverage with spider mite genes (~2000). Next steps include collecting more gene models and RNAseq data to further train the splice to gene prediction programs. A relative good quality of B. yothersi genome assembly was obtained and the gene prediction quality was improved using evidence from spider mite alignments. The annotated B. yothersi genome constitutes a valuable genomic resources to investigate the biology and the rare thelytoky system of the complex mite-virus-plant interaction. Financial Support: CNPq, Fapesp, Embrapa.

Genome annotation of the flat mite Brevipalpus yothersi Baker (Tenuipalpidae).

Genome annotation of the flat mite Brevipalpus yothersi Baker (Tenuipalpidae).

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