ABDALLA, A. L.

DUNLAP, C.

LOUVANDINI, H.

MENDES, R.

ROMAGNOLI, E. M.

TSAI, S. M.

English

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Dynamics of the sheep rumen microbiome and its relationship with the degradation of biomass Emiliana Manesco Romagnoli*1, Christopher Dunlap2 , Helder Louvandini3 , Tsai Siu Miu3 , Adibe Luiz Abdalla3, Rodrigo Mendes4 1 Luiz de Queiroz College of Agricultura/, University of Sao Paulo (ESALQIUSP), Brazil, 2National Center for Agricultura/ Utilization Research, United States Department ofAgricultura/, United States, 3Center for Nuclear Energy in Agriculture, University of SaoPaulo, Brazil, 4Embrapa Environment, Laboratory of Environmental Microbiology, Brazil Considering diet as a modulator of the ruminai microbiome, this work aimed to investigate the impact-of sugarcane bagasse on the composition and function of microbial species inhabiting the sheep ( Ovis aires) rumen. Six cannulated male animais were used in the experiment, where three individuais were fed on a diet consisting of 70% forage and 30% concentrate (control treatment), and three were fed on a similar diet, but with sugarcane bagasse replacing 14% of the forage portion (bagasse treatment). The ruminai contents, i.e., liquid and fiber, were sampled every two weeks during 60 days. We assessed the structure of microbial communities by sequencing regions of bacterial 16S rRNA genes and the fungai intergenic region (ITS2). The 16S rRNA amplicon sequencing analyses revealed a microbial community dominated mainly by Firmicutes and Bacteroidetes throughout the experimental period. The genera Prevotella and Ruminococcus accounted for 20% and 4% of the bacterial community of rumen, respectively. ln the fungai community, the phylum Neocallimastigomycota accounted for 91 % of sequences and the main genera adhered on the ruminai fiber were Piromyces, Neocallimastix, Orpinomyces, Anaeromyces, Caecomyces, and Cyllamyces. Furthermore, metagenomic data analysis showed a significant increase in the frequency of enzymes, such as a-1,4-glucan, a-galactosidase, endo-1,4-13-xylanase, 13-xylosidase, xylose isomerase, cellobiose phosphorylase and a- N-arabinofuranosidasein the bagasse treatment. The results of this study demonstrats the potential to increasethe discovery and/or recovery of biomass degrading enzymes from ruminants, as well asthe possibility to enrich specific microbial groups or functions in the sheep rumen bychanging diet.830 PS23 - Applying -omics approaches to understand microbial functioning / 

Dynamics of sheep rumen microbiome and its relationship with the degradation of biomass.

http://www.alice.cnptia.embrapa.br/bitstream/doc/1071451/1/2016RA088.pdf

Dynamics of sheep rumen microbiome and its relationship with the degradation of biomass.

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