CATARINO, A. D. M.

EIRAS, M.

FAJARDO, T. V. M.

RIBEIRO, G. P.

English

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CD ROM :: 46º Congresso Brasileiro de Fitopatologia Resumo:371-2371-2Detection and partial molecular characterization of isolates of Grapevine leafroll-associatedvirus 4 and Grapevine rupestris vein feathering virus(Detecção e caracterização molecular parcial de isolados de Grapevine leafroll-associated virus 4e Grapevine rupestris vein feathering virus) Autores: CATARINO, A. D. M. (UFRPE - Universidade Federal Rural de Pernambuco / CNPUV -Embrapa Uva e Vinho) FAJARDO, T. V. M. - thor.fajardo@embrapa.br (CNPUV - Embrapa Uvae Vinho) ; EIRAS, M. (IB - Instituto Biológico de São Paulo) ; RIBEIRO, G. P. (UFRPE -Universidade Federal Rural de Pernambuco) ; NICKEL, O. (CNPUV - Embrapa Uva e Vinho)ResumoReductions in productivity and quality of grapes and shortening of the productive life of the vineyard aredamages caused by grapevine viruses. The aim of this work was to detect and partially characterize twoisolates  of  grapevine  viruses.  Brazilian  isolates  of  Grapevine  leafroll-associated  virus  4  (GLRaV-4,Closteroviridae,  Ampelovirus)  and  Grapevine  rupestris  vein  feathering  virus  (GRVFV,  Tymoviridae,Marafivirus) were detected by RT-PCR and the DNA fragments of these viruses were RT-PCR-amplified,cloned,  sequenced and aligned.  The nucleotide (302 nt  of  partial  HSP70)  and the amino acid (100 aa)sequences of  the RB (GenBank accession code KC202814) and ME (KC202815)  isolates of  GLRaV-4showed 99% of  nucleotide  identity  when  compared  with  o her  GLRaV-4  isolates  from GenBank.  Thenucleotide (474 nt of partial coat protein) and the amino acid (158 aa) sequences of the MER (KC815703)and SEM (KC815704) isolates of GRVFV showed 87% of nucleotide identity when compared with othertwo GRVFV sequences from GenBank (AY706994, AY128949). GLRaV-4 and GRVFV were detected byTaqMan real-time RT-PCR respectively in 75% and 8% out of 101 samples of different cultivars from theBrazilian  Northeastern  region.  In  Brazil,  GLRaV-4  and  GRVFV are  associated  with  grapevine  leafrollcomplex  and  with  Tymoviridae  grapevine  infecting  viruses,  respectively,  while  other  grapevine  leafrollviruses (GLRaV-1, GLRaV-2, GLRaV-3, GLRaV-5 and GLRaV-6), and grapevine fleck and related viruses(Grapevine fleck virus, GFkV) had already been detected in grapevines.Apoio: EmbrapaCD ROM :: 46º Congresso Brasileiro de Fitopatologia file:///E|/resumos/R0371-2.html1 de 1 28/10/2013 16:23

Detection and partial molecular characterization of isolates of Grapevine leafroll-associated virus 4 and Grapevine rupestris vein feathering virus.

http://www.alice.cnptia.embrapa.br/bitstream/doc/979484/1/CDROM46CongressoBrasileirodeFitopatologiaResumo3712.pdf

Detection and partial molecular characterization of isolates of Grapevine leafroll-associated virus 4 and Grapevine rupestris vein feathering virus.

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