Nowadays the global viticulture scenario has being verifying high levels of an event known as ?young grapevine decline? related to various diseases, such as Esca, Petri and Black Foot. On the grapevine plant propagation, the process of callus formation is crucial to ensure protection against the infection of these fungus during the rooting process on the field. In the heated rooms, the formation of callus on the rootstock cuttings act like a preventive barrier against a possible infection by these fungus. The callusing process (called ?forcing?) is based on the usage of substrates for the maintenance of humidity of the cuttings and for providing optimum conditions of temperature and relative humidity in the dark chamber