KOCH, K. E.

SOUSA, S. M. de

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Resumos do GENÉTICA 2016 - 62º Congresso Brasileiro de Genética • 11 a 14 de setembro de 2016Hotel Glória • Caxambu • MG • Brasil www.sbg.org.br - ISBN 978-85-89109-06-2 Brazilian-International Congress of GeneticsMaize aldo-keto reductase family members expression is consistent with its multiple roles de Sousa, SM1, Koch, K.E.21 Embrapa Milho e Sorgo, Sete Lagoas, MG; 2University of Florida, Gainesville, FL, USA. Keywords: aldose reductase, gene expression, western blot, real time PCR, aldehydes.Aldo-keto reductases (AKRs) can catalyze diverse reactions and may have multiple roles, from antioxidant pathways to signaling pathway. Most AKR superfamily members possess broad substrate specificities, making the unequivocal determination of the physiological function of uncharacterized AKRs difficult. In this study, we presented and characterized eight maize putative AKRs. In order to analyze in detail the expression of maize AKRs at the protein level we raised polyclonal antisera against the recombinant maize AR and used it in western blot assays. We also designed specific primers for each of the eight putative maize ARs to analyze their gene expression in different tissues and development times. The analysis of different maize tissues showed reaction with several polypeptides. The amount of each polypeptide also appeared to vary among tissues, consistent with potentially different roles for the AR-like polypeptides.  Data on western blots were consistent with predicted molecular weights of the AR family members as well as their gene expression patterns.  These results showed that maize AKRs have different roles, depending on the tissues and developmental stage, shedding a light on the illusive AKR pathway. Fonte de financiamento: USDA-NRI, FAPEMIG, EMBRAPA

Maize aldo-keto reductase family members expression is consistent with its multiple roles.

http://www.alice.cnptia.embrapa.br/bitstream/doc/1056375/1/Maizealdo.pdf

Maize aldo-keto reductase family members expression is consistent with its multiple roles.

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