FONSECA, L.

GUIMARAES, L.

MARRIEL, I.

MELO, I.

OLIVEIRA, C.

OLIVEIRA, J. C.

RAMOS, T.

REIS, D.

English

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MA-0451NITROGEN CONCENTRATION IN PLANT AND POPULATION DIAZOTROPHSIN MAIZE RHIZOSPHERE INFLUENCED BY AZOSPIRILLUM APPLICATIONMETHODD Reis , L Fonseca , T Ramos , JC Oliveira , I Melo , C Oliveira , L Guimarães , I Marriel Federal University of São João del Rei, Brazil. Embrapa Maize and Sorghum, BrazilThe use of inoculants based on nitrogen-fixing bacteria technology in agriculture is a viable alternative toreduce  dependence  on  imported  synthetic  fertilizers,  and  mitigate  the  economic  and  environmentalimpacts.  In  the  case  of  maize  crops,  the  efficiency  of  bacteria  plant  interaction  -  also  depends  onoptimization. This work aimed evaluate the influence of Azospirillum brasilense application methods on Nuptake and diazotrophic population of the rhizosphere.  The experiment was conducted in the agriculturalyear 2012/2013 in soil  classified as dystrophic Hapludox in experimental area of Embrapa Maize andSorghum, Sete Lagoas, Minas Gerais, Brazil. Seven methods of application of inoculant were compared:furrow application, via spray, the seed; foliar application to 10 days after germination (DAG 10); furrow +foliar  to 20 DAG; seed + 20 to DAG; foliar  10 to DAG and 20; and control  without inoculation), andtopdressing nitrogen doses (0, 40 and 80 kg ha-1 N). Using randomized block design with split plot, withlevels of N in the plots and application of methods in sub-plots.The inoculant constituted a mixture of twostrains of Azospirillum brasilense. For applications via spraying was used a suspension mixture of twostrains of cells (108 UFC / mL). For the seed inoculation, was used the solid inoculant using charcoal ascarrier, containing the equivalent cell density. In the flowering stage, three plants were collected per plotfor nutrientes determining and rhizosphere soil samples to quantify the most probable number (MNP) ofdiazotrophic  microorganisms.  The  plants  nutrient  content  were  carried  out  in  an  optical  emissionspectrometry plasma. Soil samples were subjected to serial dilution and aliquot was transferred for threetubes with nitrogen-free (Nfb) semi-solid. The positive tubes were used for estimation of MNP from ofMcCrady table. There are significant differences in concentration of N and diazotrophic population in therhizosphere of plants. The application methods of inoculation in the furrow via spraying, via seed and seed+ spraying 20 DAG showed similar agronomic efficiency, and superior to other method. On the other hand,larger populations of rhizosphere diazotrophic were observed in 10 DAG spray methods (59 x 103) andspraying DAG + 10 20 dkg (51 x 103) depending on the applied N rate. It is concluded that the inoculationmethod influence the absorption of nutrients and diazotrophic population present in the rhizosphere ofplants.1 1 1 1 1 1 2 212XXIII Congreso Latinoamericano de Microbiología http://alam-cam2016.posterselectronicos.com/Abstract/55e4aa09-6243...2 de 3 21/11/2016 10:36

Nitrogen concentration in plant and population diazotrophs in maize rhizosphere influenced by azospirillum application method.

http://www.alice.cnptia.embrapa.br/bitstream/doc/1056699/1/Nitrogenconcentration.pdf

Nitrogen concentration in plant and population diazotrophs in maize rhizosphere influenced by azospirillum application method.

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